Experiment setAIT072 for Pseudomonas fluorescens FW300-N2E2
L-Serine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + L-Serine (20 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
Growth: about 3.5 generations
By: Mark on 1/21/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 33 genes in this experiment
For nitrogen source L-Serine in Pseudomonas fluorescens FW300-N2E2
For nitrogen source L-Serine across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Alanine and aspartate metabolism
- Cysteine metabolism
- Lipopolysaccharide biosynthesis
- Nitrogen metabolism
- Purine metabolism
- Puromycin biosynthesis
- Glutamate metabolism
- Valine, leucine and isoleucine biosynthesis
- Lysine biosynthesis
- Arginine and proline metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Novobiocin biosynthesis
- Cyanoamino acid metabolism
- Glutathione metabolism
- Nucleotide sugars metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Sphingolipid metabolism
- 1,4-Dichlorobenzene degradation
- One carbon pool by folate
- Carbon fixation in photosynthetic organisms
- Nicotinate and nicotinamide metabolism
- Folate biosynthesis
- Caprolactam degradation
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Drug metabolism - other enzymes
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: