Experiment setAIT071 for Pseudomonas fluorescens FW300-N2E2

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L-Glutamic acid monopotassium salt monohydrate nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_64 -6.1 -4.2 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_4597 -6.0 -7.1 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_4825 -5.8 -6.9 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3253 -5.8 -5.6 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_3783 -5.8 -5.6 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4596 -5.5 -8.4 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3842 -5.3 -5.2 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_3248 -5.2 -6.1 Probable transmembrane protein compare
Pf6N2E2_3630 -5.0 -7.6 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_71 -4.9 -6.7 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3782 -4.8 -3.3 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3610 -4.7 -7.2 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_77 -4.7 -6.3 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3841 -4.6 -5.5 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_2073 -4.6 -5.5 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3940 -4.6 -11.4 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4047 -4.6 -6.2 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4826 -4.5 -8.6 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_5177 -4.5 -12.2 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3932 -4.4 -6.3 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_66 -4.4 -10.4 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_5176 -4.3 -9.6 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3763 -4.3 -4.2 hypothetical protein compare
Pf6N2E2_5175 -4.3 -13.7 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3839 -4.2 -5.0 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_4048 -4.2 -8.3 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_50 -4.1 -9.8 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_302 -4.0 -7.8 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_4803 -4.0 -2.7 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_2074 -3.9 -2.6 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_3252 -3.9 -9.6 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3762 -3.9 -9.2 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_5258 -3.9 -9.9 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_2465 -3.8 -8.9 Cys regulon transcriptional activator CysB compare
Pf6N2E2_4759 -3.7 -16.5 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_5950 -3.6 -4.9 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_4761 -3.5 -13.2 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_5728 -3.4 -5.5 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_63 -3.3 -5.6 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_3251 -3.3 -10.9 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_4564 -3.2 -7.1 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_1705 -3.2 -2.5 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_5257 -3.1 -9.2 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_4763 -2.9 -9.9 Biotin synthesis protein BioC compare
Pf6N2E2_4762 -2.8 -4.6 Biotin synthesis protein BioH compare
Pf6N2E2_4206 -2.7 -2.9 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_5579 -2.7 -6.9 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_4764 -2.7 -6.0 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_4418 -2.6 -1.7 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_5001 -2.4 -3.0 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_3170 -2.3 -6.6 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_3938 -2.3 -10.8 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_4009 -2.2 -10.7 GGDEF domain protein compare
Pf6N2E2_4631 -2.1 -7.3 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_3826 -2.0 -5.6 Nitrogen regulation protein NR(I) compare
Pf6N2E2_3984 -2.0 -9.5 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_5527 -2.0 -3.1 Cold shock protein CspC compare
Pf6N2E2_4797 -1.9 -1.9 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_51 -1.9 -6.7 hypothetical protein compare
Pf6N2E2_5676 -1.9 -3.1 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_3460 -1.9 -2.1 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_4638 -1.9 -3.2 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_5252 -1.8 -2.1 FIG00956396: hypothetical protein compare
Pf6N2E2_3461 -1.8 -2.0 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_5242 -1.8 -2.3 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_987 -1.8 -2.8 hypothetical protein compare
Pf6N2E2_3947 -1.7 -1.9 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_3696 -1.7 -1.7 hypothetical protein compare
Pf6N2E2_5006 -1.6 -5.7 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_4416 -1.6 -2.8 Tyrosine recombinase XerC compare
Pf6N2E2_3060 -1.6 -3.6 Outer membrane protein H precursor compare
Pf6N2E2_5190 -1.6 -7.4 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_5316 -1.6 -2.3 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_5103 -1.5 -1.6 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_3261 -1.5 -4.1 hypothetical protein compare
Pf6N2E2_2473 -1.5 -1.4 Major outer membrane lipoprotein I compare
Pf6N2E2_3171 -1.5 -1.7 hypothetical protein compare
Pf6N2E2_2513 -1.4 -4.5 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_2753 -1.4 -2.7 HtrA protease/chaperone protein compare
Pf6N2E2_4399 -1.4 -5.1 GGDEF domain/EAL domain protein compare
Pf6N2E2_2371 -1.4 -2.8 Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE) compare
Pf6N2E2_3534 -1.4 -1.9 Cobalamin biosynthesis protein CobG compare
Pf6N2E2_2999 -1.4 -3.0 probable tail fiber assembly protein compare
Pf6N2E2_2402 -1.3 -2.1 hypothetical protein compare
Pf6N2E2_3027 -1.3 -1.7 Baseplate assembly protein V compare
Pf6N2E2_2277 -1.3 -2.0 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_3569 -1.3 -1.8 hypothetical protein compare
Pf6N2E2_16 -1.3 -1.8 hypothetical protein compare
Pf6N2E2_3264 -1.3 -2.5 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_2072 -1.2 -2.5 hypothetical protein compare
Pf6N2E2_1870 -1.2 -2.1 hypothetical protein compare
Pf6N2E2_4025 -1.2 -2.0 hypothetical protein compare
Pf6N2E2_2488 -1.2 -1.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2852 -1.2 -6.3 GGDEF domain/EAL domain protein compare
Pf6N2E2_4467 -1.2 -3.5 hypothetical protein compare
Pf6N2E2_4773 -1.2 -1.1 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_2518 -1.1 -1.5 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_2065 -1.1 -2.4 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_1656 -1.1 -1.6 hypothetical protein compare
Pf6N2E2_2258 -1.1 -6.7 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_5406 -1.1 -1.9 FIG00958224: hypothetical protein compare
Pf6N2E2_5727 -1.1 -4.7 FIG00955360: hypothetical protein compare
Pf6N2E2_127 -1.1 -2.0 AttE component of AttEFGH ABC transport system compare
Pf6N2E2_3198 -1.1 -1.7 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_1147 -1.1 -1.8 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) compare
Pf6N2E2_3134 -1.1 -1.1 hypothetical protein compare
Pf6N2E2_4987 -1.1 -1.3 MaoC-like domain protein compare
Pf6N2E2_4162 -1.1 -2.7 DNA polymerase I (EC 2.7.7.7) compare
Pf6N2E2_5156 -1.1 -2.0 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_1315 -1.1 -1.4 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) compare
Pf6N2E2_2264 -1.0 -3.0 NADPH dependent preQ0 reductase (EC 1.7.1.13) compare
Pf6N2E2_2261 -1.0 -4.5 VacJ-like lipoprotein precursor compare
Pf6N2E2_2885 -1.0 -4.4 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_3100 -1.0 -1.7 FIG00953063: hypothetical protein compare
Pf6N2E2_3260 -1.0 -3.3 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_178 -1.0 -1.1 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_3351 -1.0 -3.1 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_3698 -1.0 -2.8 Nicotinamidase family protein YcaC compare
Pf6N2E2_3257 -1.0 -2.7 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_5153 -1.0 -3.5 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_3847 -1.0 -2.1 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_189 -1.0 -2.3 regulatory protein, putative compare
Pf6N2E2_724 -1.0 -1.9 Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family) compare
Pf6N2E2_5910 -1.0 -2.4 Flp pilus assembly protein RcpC/CpaB compare
Pf6N2E2_5746 -1.0 -2.2 Cobalamin synthase compare
Pf6N2E2_4362 -1.0 -1.8 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_2080 -1.0 -1.0 hypothetical protein compare
Pf6N2E2_605 -1.0 -2.2 FIG002465: BNR repeat protein compare
Pf6N2E2_1606 -1.0 -4.0 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_2855 -1.0 -1.9 DGPFAETKE compare
Pf6N2E2_3872 -1.0 -2.9 hypothetical protein compare
Pf6N2E2_5640 -1.0 -1.7 FIG00955597: hypothetical protein compare
Pf6N2E2_3516 -1.0 -1.1 Probable transmembrane protein compare
Pf6N2E2_3825 -0.9 -2.6 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_2013 -0.9 -1.1 hypothetical protein compare
Pf6N2E2_2217 -0.9 -2.8 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_4276 -0.9 -2.3 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_2062 -0.9 -2.2 FIG00955561: hypothetical protein compare
Pf6N2E2_2064 -0.9 -2.9 Cobalt-precorrin-3b C17-methyltransferase compare
Pf6N2E2_2071 -0.9 -2.3 hypothetical protein compare
Pf6N2E2_2588 -0.9 -2.5 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_5014 -0.9 -1.0 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_2017 -0.9 -2.2 DNA-binding protein compare
Pf6N2E2_2688 -0.9 -2.8 YD repeat protein compare
Pf6N2E2_2790 -0.9 -2.4 Helix-turn-helix motif compare
Pf6N2E2_1838 -0.9 -2.2 6-hexanolactone hydrolase compare
Pf6N2E2_3908 -0.9 -3.9 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2480 -0.9 -1.5 FIG00957636: hypothetical protein compare
Pf6N2E2_4171 -0.9 -2.9 hypothetical protein compare
Pf6N2E2_5943 -0.8 -1.7 hypothetical protein compare
Pf6N2E2_4077 -0.8 -1.2 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_3338 -0.8 -1.6 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_5972 -0.8 -2.3 hypothetical protein compare
Pf6N2E2_2281 -0.8 -2.0 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_5740 -0.8 -2.5 Adenosylcobinamide-phosphate synthase (EC 6.3.1.10) compare
Pf6N2E2_21 -0.8 -2.7 Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis compare
Pf6N2E2_4636 -0.8 -4.1 periplasmic binding protein, putative compare
Pf6N2E2_2778 -0.8 -2.7 Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10) compare
Pf6N2E2_4824 -0.8 -2.1 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_2756 -0.8 -3.8 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_4273 -0.8 -3.8 ATP-dependent DNA helicase UvrD/PcrA compare
Pf6N2E2_2068 -0.8 -4.1 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) compare
Pf6N2E2_2605 -0.8 -3.0 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_6100 -0.8 -1.9 tRNA-Val-TAC compare
Pf6N2E2_3349 -0.8 -0.9 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_1694 -0.8 -1.2 FMN reductase (EC 1.5.1.29) compare
Pf6N2E2_3536 -0.8 -3.6 Cobalt-precorrin-6 synthase, anaerobic compare
Pf6N2E2_3183 -0.8 -2.2 Believed to be involved in assembly of Fe-S clusters compare
Pf6N2E2_5340 -0.8 -2.8 Stringent starvation protein A compare
Pf6N2E2_2791 -0.8 -2.3 FIG00954883: hypothetical protein compare
Pf6N2E2_2253 -0.8 -2.4 hypothetical protein compare
Pf6N2E2_649 -0.8 -1.4 General secretion pathway protein L compare
Pf6N2E2_2940 -0.8 -3.5 OmpA family protein compare
Pf6N2E2_5508 -0.8 -1.5 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_5545 -0.8 -1.5 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_4307 -0.8 -1.8 Type VI secretion lipoprotein/VasD compare
Pf6N2E2_65 -0.8 -2.1 SAM-dependent methyltransferase YafE (UbiE paralog) compare
Pf6N2E2_3406 -0.8 -2.6 putative ABC transporter ATP-binding protein compare
Pf6N2E2_4459 -0.8 -1.1 Transcriptional regulator, GntR family compare
Pf6N2E2_1525 -0.8 -2.2 Universal stress protein family 5 compare
Pf6N2E2_4278 -0.8 -4.9 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_5411 -0.8 -2.0 FIG00955494: hypothetical protein compare
Pf6N2E2_4372 -0.8 -2.3 FIG00460773: hypothetical protein compare
Pf6N2E2_2499 -0.8 -1.5 Competence protein ComEA helix-hairpin-helix region precursor compare
Pf6N2E2_75 -0.7 -1.6 Colicin V production protein compare
Pf6N2E2_5745 -0.7 -3.5 Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73) compare
Pf6N2E2_99 -0.7 -2.6 hypothetical protein compare
Pf6N2E2_4272 -0.7 -1.8 putative membrane protein compare
Pf6N2E2_350 -0.7 -1.7 Gfa-like protein compare
Pf6N2E2_5571 -0.7 -0.9 hypothetical protein compare
Pf6N2E2_4454 -0.7 -2.9 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_3199 -0.7 -3.0 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_5052 -0.7 -1.3 Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family compare
Pf6N2E2_3571 -0.7 -2.7 Urease accessory protein UreF compare
Pf6N2E2_1718 -0.7 -1.4 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) compare
Pf6N2E2_5471 -0.7 -4.2 Transcriptional activator MetR compare
Pf6N2E2_1403 -0.7 -1.4 Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL compare
Pf6N2E2_2152 -0.7 -0.8 hypothetical protein compare
Pf6N2E2_3062 -0.7 -2.6 Membrane-associated zinc metalloprotease compare
Pf6N2E2_1717 -0.7 -3.8 Anthranilate synthase, aminase component (EC 4.1.3.27) compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N2E2 in nitrogen source experiments

For nitrogen source L-Glutamic acid monopotassium salt monohydrate across organisms