Experiment setAIT069 for Pseudomonas fluorescens FW300-N2E2

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L-Lysine nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_4597 -6.2 -4.3 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3610 -6.0 -4.1 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_71 -6.0 -4.1 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_4826 -6.0 -4.1 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_64 -5.9 -4.0 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3842 -5.5 -3.8 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_3630 -5.5 -5.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4825 -5.4 -6.5 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_4596 -5.3 -8.1 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_5006 -5.2 -7.1 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_4777 -5.0 -9.0 5-aminopentanamidase (EC 3.5.1.30) (from data) conserved
Pf6N2E2_4779 -5.0 -6.8 Transcriptional regulator, AsnC family conserved
Pf6N2E2_5176 -5.0 -5.9 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_4759 -4.9 -11.8 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_4764 -4.9 -5.8 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_3248 -4.8 -5.7 Probable transmembrane protein compare
Pf6N2E2_3751 -4.7 -11.0 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4077 -4.7 -3.3 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_3253 -4.7 -6.4 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4564 -4.7 -10.8 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_3252 -4.6 -7.0 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_4047 -4.6 -4.5 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_3940 -4.6 -9.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_66 -4.5 -9.4 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_5177 -4.4 -11.3 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_4987 -4.4 -2.0 MaoC-like domain protein compare
Pf6N2E2_3783 -4.4 -6.0 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4279 -4.4 -12.2 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_3752 -4.3 -19.2 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_2588 -4.3 -4.2 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_3825 -4.3 -5.1 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_2897 -4.3 -9.9 Glucokinase (EC 2.7.1.2) compare
Pf6N2E2_2073 -4.3 -5.1 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_5175 -4.3 -11.9 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_4763 -4.1 -10.7 Biotin synthesis protein BioC compare
Pf6N2E2_3932 -4.1 -5.4 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_2885 -4.0 -6.1 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_4048 -4.0 -7.4 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_4631 -4.0 -11.2 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_3826 -4.0 -6.0 Nitrogen regulation protein NR(I) compare
Pf6N2E2_4761 -4.0 -11.7 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_302 -3.9 -7.4 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_77 -3.8 -5.8 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3839 -3.8 -4.5 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_4778 -3.8 -15.3 Lysine 2-monooxygenase (EC 1.13.12.2) (from data) conserved
Pf6N2E2_4278 -3.8 -12.1 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_2074 -3.8 -2.3 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_2863 -3.7 -8.1 Gluconate permease compare
Pf6N2E2_3941 -3.7 -4.3 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_2507 -3.7 -7.0 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_658 -3.6 -12.6 sensor histidine kinase compare
Pf6N2E2_4762 -3.6 -2.7 Biotin synthesis protein BioH compare
Pf6N2E2_50 -3.6 -9.2 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_5950 -3.5 -4.2 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_3661 -3.5 -4.8 hypothetical protein compare
Pf6N2E2_3782 -3.5 -4.1 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_4611 -3.4 -4.0 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_2753 -3.4 -2.3 HtrA protease/chaperone protein compare
Pf6N2E2_2258 -3.2 -11.7 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_2510 -3.2 -5.3 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_3841 -3.2 -4.7 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3460 -3.1 -2.1 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_63 -3.1 -4.6 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_3869 -3.0 -11.5 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_4372 -3.0 -4.9 FIG00460773: hypothetical protein compare
Pf6N2E2_5150 -2.9 -6.7 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_3346 -2.9 -6.6 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_613 -2.9 -2.2 lipoprotein, putative compare
Pf6N2E2_5579 -2.9 -8.1 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_3072 -2.8 -11.3 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_2962 -2.8 -2.4 ABC transporter for L-Lysine, ATPase component (from data) compare
Pf6N2E2_4277 -2.8 -8.7 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_5553 -2.8 -7.2 Quinolinate synthetase (EC 2.5.1.72) compare
Pf6N2E2_3170 -2.6 -7.1 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_5160 -2.6 -2.0 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_5014 -2.5 -1.6 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_4638 -2.5 -1.7 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_3659 -2.5 -6.3 Protein fixF compare
Pf6N2E2_2899 -2.4 -6.0 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_5156 -2.4 -2.3 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_3658 -2.4 -7.9 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_3665 -2.4 -1.5 serine/threonine protein kinase compare
Pf6N2E2_3251 -2.4 -8.8 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_2465 -2.4 -4.7 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2117 -2.3 -12.7 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) compare
Pf6N2E2_3938 -2.3 -11.0 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_5086 -2.3 -1.4 hypothetical protein compare
Pf6N2E2_2508 -2.3 -4.2 hypothetical protein compare
Pf6N2E2_4803 -2.2 -2.4 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_2218 -2.1 -4.4 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_5728 -2.1 -4.6 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_3908 -2.1 -5.7 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2281 -2.1 -2.4 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_1381 -2.1 -7.4 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf6N2E2_5527 -2.1 -2.7 Cold shock protein CspC compare
Pf6N2E2_2506 -2.1 -12.1 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_4187 -2.1 -3.9 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_1705 -2.0 -1.9 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_3984 -2.0 -9.0 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_1400 -1.9 -10.4 Sensory box histidine kinase/response regulator compare
Pf6N2E2_2186 -1.9 -5.4 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_2864 -1.9 -2.8 Gluconokinase (EC 2.7.1.12) compare
Pf6N2E2_2959 -1.9 -8.2 ABC transporter for L-Lysine, permease component 1 (from data) conserved
Pf6N2E2_1056 -1.8 -1.1 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_5153 -1.7 -5.1 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_5723 -1.7 -8.9 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_2752 -1.7 -7.4 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_4009 -1.7 -7.8 GGDEF domain protein compare
Pf6N2E2_4057 -1.7 -1.1 lipoprotein, putative compare
Pf6N2E2_3942 -1.7 -7.9 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_3663 -1.7 -6.1 Glycosyl transferase compare
Pf6N2E2_5536 -1.7 -1.6 Holliday junction DNA helicase RuvA compare
Pf6N2E2_2960 -1.7 -9.9 ABC transporter for L-Lysine, permease component 2 (from data) conserved
Pf6N2E2_4352 -1.7 -2.5 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_5242 -1.7 -2.1 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_2674 -1.6 -1.5 FIG00953856: hypothetical protein compare
Pf6N2E2_2688 -1.6 -4.1 YD repeat protein compare
Pf6N2E2_2219 -1.6 -1.9 Cell division trigger factor (EC 5.2.1.8) compare
Pf6N2E2_2274 -1.6 -5.0 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_3849 -1.5 -2.9 FIG00954739: hypothetical protein compare
Pf6N2E2_1187 -1.5 -5.9 Probable nikkomycin biosynthesis protein, carboxylase compare
Pf6N2E2_178 -1.5 -1.3 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_3033 -1.5 -1.5 hypothetical protein compare
Pf6N2E2_3282 -1.5 -2.2 FIG005080: Possible exported protein compare
Pf6N2E2_4454 -1.5 -4.9 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_3868 -1.5 -6.5 Transcriptional regulator, LysR family compare
Pf6N2E2_3461 -1.5 -1.6 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_1932 -1.5 -3.8 Transcriptional regulator, IclR family compare
Pf6N2E2_5061 -1.4 -3.4 Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25) compare
Pf6N2E2_5663 -1.4 -2.9 L-Arginine ABC transporter, ATPase component (from data) compare
Pf6N2E2_1186 -1.4 -3.7 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_3885 -1.4 -2.5 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_4516 -1.4 -6.7 Putrescine transport system permease protein PotH (TC 3.A.1.11.2) compare
Pf6N2E2_2675 -1.4 -1.2 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_5001 -1.4 -1.5 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_1596 -1.4 -1.6 Periplasmic nitrate reductase component NapD compare
Pf6N2E2_5785 -1.4 -1.8 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5425 -1.3 -1.1 hypothetical protein compare
Pf6N2E2_3257 -1.3 -3.0 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_2890 -1.3 -4.2 Glucose ABC transport system, inner membrane component 2 compare
Pf6N2E2_2852 -1.3 -5.8 GGDEF domain/EAL domain protein compare
Pf6N2E2_5316 -1.3 -1.7 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_2958 -1.3 -5.7 ABC transporter for L-Lysine, periplasmic substrate-binding component (from data) conserved
Pf6N2E2_4013 -1.3 -7.2 5-aminovalerate transaminase (EC 2.6.1.48) (from data) conserved
Pf6N2E2_5339 -1.3 -4.6 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_3027 -1.3 -1.4 Baseplate assembly protein V compare
Pf6N2E2_4984 -1.3 -1.8 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_4515 -1.3 -6.1 Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2) compare
Pf6N2E2_3677 -1.3 -7.5 Sensory box/GGDEF family protein compare
Pf6N2E2_3554 -1.3 -6.0 GCN5-related N-acetyltransferase compare
Pf6N2E2_4276 -1.3 -4.1 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_2375 -1.3 -2.1 Response regulator NasT compare
Pf6N2E2_893 -1.3 -2.5 hypothetical protein compare
Pf6N2E2_2527 -1.3 -4.0 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_2292 -1.3 -2.7 Universal stress protein family COG0589 compare
Pf6N2E2_5714 -1.2 -1.8 Flavoprotein WrbA compare
Pf6N2E2_5780 -1.2 -6.4 Periplasmic protease compare
Pf6N2E2_2514 -1.2 -3.6 regulator of length of O-antigen component of lipopolysaccharide chains compare
Pf6N2E2_2217 -1.2 -3.2 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_4513 -1.2 -6.8 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) conserved
Pf6N2E2_2340 -1.2 -2.7 Transcriptional regulator, GntR family compare
Pf6N2E2_4499 -1.2 -2.2 protein of unknown function DUF86 compare
Pf6N2E2_5337 -1.2 -3.6 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_4517 -1.2 -3.0 Putrescine transport system permease protein PotI (TC 3.A.1.11.2) compare
Pf6N2E2_4498 -1.2 -7.6 Outer membrane porin, OprD family compare
Pf6N2E2_3454 -1.1 -2.0 hypothetical protein compare
Pf6N2E2_1040 -1.1 -1.3 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_4773 -1.1 -0.9 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_3132 -1.1 -2.3 FIG00954548: hypothetical protein compare
Pf6N2E2_2758 -1.1 -7.4 L-aspartate oxidase (EC 1.4.3.16) compare
Pf6N2E2_4075 -1.1 -3.2 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_5232 -1.1 -4.7 Cell division inhibitor compare
Pf6N2E2_5544 -1.1 -5.2 TPR repeat containing exported protein; Putative periplasmic protein contains a protein prenylyltransferase domain compare
Pf6N2E2_4579 -1.1 -1.1 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_1402 -1.1 -3.9 hypothetical protein compare
Pf6N2E2_904 -1.1 -2.7 hypothetical protein compare
Pf6N2E2_648 -1.1 -1.7 General secretion pathway protein K compare
Pf6N2E2_1128 -1.1 -1.6 Two-component response regulator compare
Pf6N2E2_426 -1.1 -4.0 Arsenate reductase (EC 1.20.4.1) compare
Pf6N2E2_4453 -1.1 -7.8 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_5647 -1.1 -4.0 Transcriptional regulator, TetR family compare
Pf6N2E2_4072 -1.1 -3.2 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_5178 -1.0 -1.5 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_4636 -1.0 -4.9 periplasmic binding protein, putative compare
Pf6N2E2_4360 -1.0 -5.4 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_4824 -1.0 -2.6 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_6035 -1.0 -1.2 alpha/beta hydrolase fold compare
Pf6N2E2_5705 -1.0 -1.6 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_5133 -1.0 -2.3 FIG00953980: hypothetical protein compare
Pf6N2E2_5190 -1.0 -5.6 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_2480 -1.0 -1.1 FIG00957636: hypothetical protein compare
Pf6N2E2_4062 -1.0 -2.0 hypothetical protein compare
Pf6N2E2_1341 -1.0 -2.0 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_3095 -1.0 -0.8 hypothetical protein compare
Pf6N2E2_1440 -1.0 -2.3 Putative tRNA-m1A22 methylase compare
Pf6N2E2_5727 -1.0 -3.1 FIG00955360: hypothetical protein compare
Pf6N2E2_2981 -1.0 -2.4 FIG00956655: hypothetical protein compare
Pf6N2E2_3786 -1.0 -1.8 Twin-arginine translocation protein TatC compare
Pf6N2E2_5623 -1.0 -2.4 YciL protein compare
Pf6N2E2_3198 -1.0 -1.5 tRNA-guanine transglycosylase (EC 2.4.2.29) compare


Specific Phenotypes

For 7 genes in this experiment

For nitrogen source L-Lysine in Pseudomonas fluorescens FW300-N2E2

For nitrogen source L-Lysine across organisms