Experiment setAIT063 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB no stress 24 hours

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_2200 +1.1 2.5 RNA polymerase sigma-54 factor RpoN compare
Pf6N2E2_865 +0.9 3.0 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_6062 +0.9 5.7 2-methylcitrate synthase (EC 2.3.3.5) (from data) compare
Pf6N2E2_5720 +0.9 7.5 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_6065 +0.8 2.6 ABC transporter in pyoverdin gene cluster, periplasmic component compare
Pf6N2E2_3271 +0.8 2.0 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_5622 +0.8 2.8 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_4160 +0.8 1.7 Cytochrome c5 compare
Pf6N2E2_4974 +0.8 5.4 DedA protein compare
Pf6N2E2_2003 +0.8 2.1 hypothetical protein compare
Pf6N2E2_1981 +0.8 2.8 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_5242 +0.8 1.9 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_6099 +0.8 1.7 tRNA-Glu-TTC compare
Pf6N2E2_724 +0.8 1.5 Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family) compare
Pf6N2E2_4059 +0.7 1.4 DNA-binding protein HU-alpha compare
Pf6N2E2_2080 +0.7 1.4 hypothetical protein compare
Pf6N2E2_2083 +0.7 1.8 hypothetical protein compare
Pf6N2E2_3282 +0.7 1.5 FIG005080: Possible exported protein compare
Pf6N2E2_1213 +0.7 2.6 hypothetical protein compare
Pf6N2E2_2477 +0.7 2.5 ABC transporter ATP-binding protein YvcR compare
Pf6N2E2_2195 +0.7 2.0 cI compare
Pf6N2E2_896 +0.7 3.0 Regulatory protein, LysR:LysR, substrate-binding compare
Pf6N2E2_1759 +0.6 2.9 uncharacterized domain 1 compare
Pf6N2E2_3323 +0.6 3.3 hypothetical protein compare
Pf6N2E2_4054 +0.6 1.6 Osmotically inducible protein C compare
Pf6N2E2_48 +0.6 1.5 FIG00955661: hypothetical protein compare
Pf6N2E2_2503 +0.6 1.8 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_952 +0.6 1.4 putative exported protein compare
Pf6N2E2_5816 +0.6 1.6 Smr domain protein compare
Pf6N2E2_288 +0.6 1.5 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_1162 +0.6 1.6 Transmembrane amino acid efflux protein compare
Pf6N2E2_1779 +0.6 2.3 Positive regulator of CheA protein activity (CheW) compare
Pf6N2E2_4721 +0.6 2.3 Sarcosine oxidase gamma subunit (EC 1.5.3.1) compare
Pf6N2E2_4206 +0.6 1.6 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_4963 +0.6 1.2 Homoserine dehydrogenase (EC 1.1.1.3) compare
Pf6N2E2_3171 +0.6 1.2 hypothetical protein compare
Pf6N2E2_2927 +0.6 2.6 Putative signal peptide protein compare
Pf6N2E2_2583 +0.6 3.4 Flagellar motor switch protein FliM compare
Pf6N2E2_2156 +0.6 2.0 Putative polyketide synthase (Fragment) compare
Pf6N2E2_4300 +0.6 2.4 Uncharacterized protein similar to VCA0109 compare
Pf6N2E2_1305 +0.6 1.7 Homoprotocatechuate degradative operon repressor compare
Pf6N2E2_1425 +0.6 3.0 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) compare
Pf6N2E2_2778 +0.6 2.8 Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10) compare
Pf6N2E2_1141 +0.6 1.5 Lipid-A-disaccharide synthase (EC 2.4.1.182) compare
Pf6N2E2_5902 +0.6 2.2 hypothetical protein compare
Pf6N2E2_5499 +0.6 1.0 hypothetical protein compare
Pf6N2E2_1548 +0.6 2.2 Lactoylglutathione lyase compare
Pf6N2E2_1676 +0.6 1.3 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_5746 +0.5 2.0 Cobalamin synthase compare
Pf6N2E2_5671 +0.5 1.7 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_4464 +0.5 1.5 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_6090 +0.5 1.1 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf6N2E2_2225 +0.5 2.1 FIG00953489: hypothetical protein compare
Pf6N2E2_5794 +0.5 1.6 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_1778 +0.5 1.6 Positive regulator of CheA protein activity (CheW) compare
Pf6N2E2_4691 +0.5 1.3 hypothetical protein compare
Pf6N2E2_6164 +0.5 1.2 tRNA-Val-TAC compare
Pf6N2E2_2850 +0.5 2.3 Transporter, LysE family compare
Pf6N2E2_1735 +0.5 1.0 PhnB protein compare
Pf6N2E2_3737 +0.5 1.4 General secretion pathway protein M compare
Pf6N2E2_1472 +0.5 2.7 Cytochrome b561 compare
Pf6N2E2_2842 +0.5 1.1 hypothetical protein compare
Pf6N2E2_3014 +0.5 1.9 P2-like prophage tail protein X compare
Pf6N2E2_3033 +0.5 1.3 hypothetical protein compare
Pf6N2E2_5172 +0.5 1.6 FOG: TPR repeat compare
Pf6N2E2_2022 +0.5 2.7 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_1522 +0.5 1.8 Alcohol dehydrogenase (EC 1.1.1.1) compare
Pf6N2E2_1674 +0.5 3.0 Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) compare
Pf6N2E2_1253 +0.5 2.7 transcriptional regulator compare
Pf6N2E2_1825 +0.5 2.1 FOG: EAL domain protein compare
Pf6N2E2_3529 +0.5 1.1 Toxin A compare
Pf6N2E2_1514 +0.5 2.1 lipoprotein OprI, putative compare
Pf6N2E2_1049 +0.5 1.9 hypothetical protein compare
Pf6N2E2_1325 +0.5 1.8 FIG00954773: hypothetical protein compare
Pf6N2E2_2367 +0.5 1.1 hypothetical protein compare
Pf6N2E2_3826 +0.5 1.7 Nitrogen regulation protein NR(I) compare
Pf6N2E2_37 +0.4 1.8 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_1210 +0.4 2.5 putative aminotransferase compare
Pf6N2E2_1796 +0.4 2.0 Glycine cleavage system transcriptional activator GcvA compare
Pf6N2E2_4943 +0.4 1.9 PspA/IM30 family protein compare
Pf6N2E2_1506 +0.4 1.4 Putative alkanesulfonate metabolism utilization regulator compare
Pf6N2E2_5898 +0.4 2.1 Integral membrane protein compare
Pf6N2E2_5097 +0.4 1.6 FIG00953595: hypothetical protein compare
Pf6N2E2_2712 +0.4 2.2 Permeases of the major facilitator superfamily compare
Pf6N2E2_6035 +0.4 1.6 alpha/beta hydrolase fold compare
Pf6N2E2_2912 +0.4 0.6 FIG00954094: hypothetical protein compare
Pf6N2E2_5545 +0.4 1.7 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_574 +0.4 1.6 Transporter, LysE family compare
Pf6N2E2_49 +0.4 1.7 FIG00967200: hypothetical protein compare
Pf6N2E2_3844 +0.4 1.0 hypothetical protein compare
Pf6N2E2_4962 +0.4 1.3 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_328 +0.4 2.0 hypothetical protein compare
Pf6N2E2_2247 +0.4 1.4 Transcriptional regulator, AraC family compare
Pf6N2E2_5405 +0.4 2.5 ABC transporter for D-Alanine, ATPase component (from data) compare
Pf6N2E2_2922 +0.4 1.7 hypothetical protein compare
Pf6N2E2_906 +0.4 1.7 ISPpu14, transposase Orf2 compare
Pf6N2E2_5843 +0.4 1.4 hypothetical protein compare
Pf6N2E2_136 +0.4 1.0 Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE compare
Pf6N2E2_4570 +0.4 1.5 Cation/multidrug efflux pump compare
Pf6N2E2_4640 +0.4 1.1 hypothetical protein compare
Pf6N2E2_3037 +0.4 3.1 RNA polymerase sigma factor RpoS compare
Pf6N2E2_434 +0.4 1.3 hypothetical protein compare
Pf6N2E2_1821 +0.4 1.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_5993 +0.4 0.7 FIG00957412: hypothetical protein compare
Pf6N2E2_3139 +0.4 2.4 L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16) compare
Pf6N2E2_3868 +0.4 2.6 Transcriptional regulator, LysR family compare
Pf6N2E2_3731 +0.4 2.6 hypothetical protein compare
Pf6N2E2_1347 +0.4 1.1 Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-) compare
Pf6N2E2_6018 +0.4 1.7 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_4298 +0.4 1.3 Uncharacterized protein ImpB compare
Pf6N2E2_4843 +0.4 2.5 FIG00956941: hypothetical protein compare
Pf6N2E2_3739 +0.4 2.2 General secretion pathway protein K compare
Pf6N2E2_1396 +0.4 1.2 ABC transporter, ATP-binding protein compare
Pf6N2E2_1315 +0.4 1.0 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) compare
Pf6N2E2_3498 +0.4 1.8 FIG00953349: hypothetical protein compare
Pf6N2E2_1451 +0.4 2.0 hypothetical protein compare
Pf6N2E2_3338 +0.4 0.7 1-phosphofructokinase (EC 2.7.1.56) compare
Pf6N2E2_81 +0.4 1.0 hypothetical protein compare
Pf6N2E2_1682 +0.4 1.5 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf6N2E2_600 +0.4 1.0 Diadenosine tetraphosphatase and related serine/threonine protein phosphatases compare
Pf6N2E2_1583 +0.4 2.2 ABC-type protease/lipase transport system, ATPase and permease components compare
Pf6N2E2_3029 +0.4 1.3 Holin compare
Pf6N2E2_5168 +0.4 1.8 Regulator of competence-specific genes compare
Pf6N2E2_1353 +0.4 1.9 FIG002577: Putative lipoprotein precursor compare
Pf6N2E2_3429 +0.4 1.4 hypothetical protein compare
Pf6N2E2_5505 +0.4 1.3 hypothetical protein compare
Pf6N2E2_578 +0.4 1.4 Mobile element protein compare
Pf6N2E2_3454 +0.4 1.1 hypothetical protein compare
Pf6N2E2_4969 +0.4 0.7 hypothetical protein compare
Pf6N2E2_4584 +0.4 0.8 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
Pf6N2E2_1667 +0.4 1.1 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_1395 +0.4 2.1 Uncharacterized conserved protein compare
Pf6N2E2_3743 +0.4 2.5 General secretion pathway protein G compare
Pf6N2E2_1223 +0.4 0.9 Amino acid transporters compare
Pf6N2E2_5057 +0.4 1.5 hypothetical protein compare
Pf6N2E2_5031 +0.4 2.4 hypothetical protein compare
Pf6N2E2_1153 +0.4 1.0 small molecule metabolism; degradation; fatty acid compare
Pf6N2E2_1862 +0.4 1.6 Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10) compare
Pf6N2E2_491 +0.4 1.8 hypothetical protein compare
Pf6N2E2_159 +0.4 1.3 DNA damage resistance protein (from data) compare
Pf6N2E2_94 +0.4 2.0 Cyn operon transcriptional activator compare
Pf6N2E2_3169 +0.4 1.8 Membrane proteins related to metalloendopeptidases compare
Pf6N2E2_5730 +0.4 1.3 FIG00954480: hypothetical protein compare
Pf6N2E2_3528 +0.3 1.8 hypothetical protein compare
Pf6N2E2_3744 +0.3 0.8 General secretion pathway protein F compare
Pf6N2E2_4146 +0.3 1.1 Membrane lipoprotein lipid attachment site containing protein USSDB6D compare
Pf6N2E2_4718 +0.3 1.8 Sarcosine oxidase beta subunit (EC 1.5.3.1) compare
Pf6N2E2_4996 +0.3 1.0 LSU m3Psi1915 methyltransferase RlmH compare
Pf6N2E2_1715 +0.3 1.2 hypothetical protein compare
Pf6N2E2_1617 +0.3 0.7 COG2199: FOG: GGDEF domain compare
Pf6N2E2_6045 +0.3 0.7 hypothetical protein compare
Pf6N2E2_2948 +0.3 1.8 FIG00953202: hypothetical protein compare
Pf6N2E2_674 +0.3 2.0 Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1) compare
Pf6N2E2_5500 +0.3 1.1 MoxR-like ATPases compare
Pf6N2E2_1705 +0.3 0.7 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_5546 +0.3 2.2 Queuosine Biosynthesis QueC ATPase compare
Pf6N2E2_3275 +0.3 2.0 FOG: TPR repeat compare
Pf6N2E2_4414 +0.3 0.9 FIG00953808: hypothetical protein compare
Pf6N2E2_370 +0.3 1.4 Predicted enzyme of the cupin superfamily compare
Pf6N2E2_1287 +0.3 1.0 Mobile element protein compare
Pf6N2E2_5446 +0.3 1.6 iron-chelator utilization protein compare
Pf6N2E2_505 +0.3 1.8 hypothetical protein compare
Pf6N2E2_650 +0.3 1.8 General secretion pathway protein M compare
Pf6N2E2_652 +0.3 1.3 General secretion pathway protein E compare
Pf6N2E2_482 +0.3 1.3 Transcriptional regulator BkdR of isoleucine and valine catabolism operon compare
Pf6N2E2_1584 +0.3 1.5 Transcriptional regulator, MarR family / Acetyltransferase (GNAT) compare
Pf6N2E2_5756 +0.3 1.0 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_759 +0.3 2.2 HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217) compare
Pf6N2E2_2908 +0.3 1.6 Arginine deiminase (EC 3.5.3.6) compare
Pf6N2E2_5345 +0.3 2.5 LppC putative lipoprotein compare
Pf6N2E2_1248 +0.3 1.0 MutT/nudix family protein compare
Pf6N2E2_3428 +0.3 1.9 hypothetical protein compare
Pf6N2E2_2314 +0.3 0.7 VapB protein (antitoxin to VapC) compare
Pf6N2E2_1113 +0.3 1.0 Transcriptional regulator, TetR family compare
Pf6N2E2_3717 +0.3 1.6 Lysophospholipid acyltransferase compare
Pf6N2E2_216 +0.3 1.6 Copper-sensing two-component system response regulator CpxR compare
Pf6N2E2_5806 +0.3 1.0 hypothetical protein compare
Pf6N2E2_3680 +0.3 1.8 hypothetical protein compare
Pf6N2E2_2438 +0.3 0.4 hypothetical protein compare
Pf6N2E2_5128 +0.3 1.8 COG1496: Uncharacterized conserved protein compare
Pf6N2E2_4124 +0.3 2.6 Uncharacterized protein with LysM domain, COG1652 compare
Pf6N2E2_2421 +0.3 1.9 hypothetical protein compare
Pf6N2E2_1718 +0.3 1.0 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) compare
Pf6N2E2_3006 +0.3 1.8 Phage tail/DNA circulation protein compare
Pf6N2E2_1747 +0.3 1.4 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_963 +0.3 1.3 probable exported protein STY4558 compare
Pf6N2E2_332 +0.3 1.4 putative nucleoside-diphosphate-sugar epimerase compare
Pf6N2E2_5229 +0.3 2.2 Transcriptional regulator, MerR family, associated with photolyase compare
Pf6N2E2_4057 +0.3 0.5 lipoprotein, putative compare
Pf6N2E2_1632 +0.3 1.7 Iron(III) dicitrate transport system, periplasmic iron-binding protein FecB (TC 3.A.1.14.1) compare
Pf6N2E2_2178 +0.3 1.7 FIG00960144: hypothetical protein compare
Pf6N2E2_1675 +0.3 2.0 Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16) compare
Pf6N2E2_2594 +0.3 2.0 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_377 +0.3 0.9 hypothetical protein compare
Pf6N2E2_695 +0.3 1.3 Nitrite reductase associated c-type cytochorome NirN compare
Pf6N2E2_909 +0.3 1.6 VrlR-like protein compare
Pf6N2E2_1492 +0.3 1.1 No significant database matches compare
Pf6N2E2_5087 +0.3 1.2 Transcription elongation factor GreB-related protein compare
Pf6N2E2_1955 +0.3 2.1 daunorubicin C-13 ketoreductase XF1741 compare
Pf6N2E2_3947 +0.3 0.6 Cd(II)/Pb(II)-responsive transcriptional regulator compare


Specific Phenotypes

None in this experiment

For Pseudomonas fluorescens FW300-N2E2 in lb experiments