Experiment setAIT056 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with DEANONOate 0.000625 M

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_4153 +3.8 8.9 Sensory box histidine kinase compare
Pf6N2E2_3786 +3.6 10.3 Twin-arginine translocation protein TatC compare
Pf6N2E2_5337 +3.6 17.5 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_2428 +3.6 13.0 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_2320 +3.5 14.6 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_2318 +3.4 23.7 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_4159 +3.3 14.7 Cytochrome c4 compare
Pf6N2E2_5339 +3.2 19.0 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_2310 +3.2 22.5 Cytochrome c oxidase subunit CcoN (EC 1.9.3.1) compare
Pf6N2E2_2313 +3.2 11.0 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_5338 +3.1 19.9 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_2527 +2.9 13.5 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_2311 +2.8 18.8 Cytochrome c oxidase subunit CcoO (EC 1.9.3.1) compare
Pf6N2E2_5720 +2.8 13.1 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_2552 +2.8 11.7 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_3663 +2.7 14.1 Glycosyl transferase compare
Pf6N2E2_2317 +2.7 12.0 Putative analog of CcoH, COG3198 compare
Pf6N2E2_2022 +2.6 9.7 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_2316 +2.5 16.9 Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation compare
Pf6N2E2_2309 +2.5 5.3 hypothetical protein compare
Pf6N2E2_3782 +2.5 5.8 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_5156 +2.4 8.0 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_4120 +2.3 17.1 Trk system potassium uptake protein TrkA compare
Pf6N2E2_5520 +2.3 13.4 Sensory box histidine kinase compare
Pf6N2E2_4614 +2.2 8.8 twitching motility protein PilH compare
Pf6N2E2_5554 +2.2 8.5 FIG023103: Predicted transmembrane protein compare
Pf6N2E2_2634 +2.2 13.8 Potassium uptake protein TrkH compare
Pf6N2E2_4889 +2.0 9.5 Putative transport protein compare
Pf6N2E2_2010 +1.9 2.2 hypothetical protein compare
Pf6N2E2_2526 +1.8 6.9 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_5158 +1.8 4.1 FIG00954153: hypothetical protein compare
Pf6N2E2_1981 +1.7 4.0 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_5067 +1.7 4.5 MFS general substrate transporter compare
Pf6N2E2_3947 +1.7 2.2 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_3539 +1.6 1.7 FIG00955840: hypothetical protein compare
Pf6N2E2_1116 +1.6 1.8 Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) compare
Pf6N2E2_3785 +1.5 3.7 Twin-arginine translocation protein TatB compare
Pf6N2E2_865 +1.5 2.0 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_5157 +1.5 8.7 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2605 +1.5 7.9 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_4615 +1.5 2.5 type IV pili signal transduction protein PilI compare
Pf6N2E2_2201 +1.5 3.5 Transmembrane regulator protein PrtR compare
Pf6N2E2_5237 +1.5 3.0 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf6N2E2_4527 +1.4 3.7 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf6N2E2_4556 +1.4 3.6 FIG00953078: hypothetical protein compare
Pf6N2E2_3037 +1.4 7.1 RNA polymerase sigma factor RpoS compare
Pf6N2E2_3301 +1.3 9.1 ABC transporter, ATP-binding protein compare
Pf6N2E2_4579 +1.3 2.8 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_3826 +1.3 4.4 Nitrogen regulation protein NR(I) compare
Pf6N2E2_3543 +1.3 8.0 Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB compare
Pf6N2E2_817 +1.3 2.6 Protein-glutamate methylesterase (EC 3.1.1.61) compare
Pf6N2E2_4987 +1.3 1.9 MaoC-like domain protein compare
Pf6N2E2_5758 +1.3 3.3 FIG00957314: hypothetical protein compare
Pf6N2E2_3825 +1.3 3.3 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_2106 +1.3 2.7 hypothetical protein compare
Pf6N2E2_3899 +1.3 7.7 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_6065 +1.3 3.0 ABC transporter in pyoverdin gene cluster, periplasmic component compare
Pf6N2E2_5671 +1.2 2.5 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_3183 +1.2 3.3 Believed to be involved in assembly of Fe-S clusters compare
Pf6N2E2_4422 +1.2 3.8 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_3305 +1.2 4.2 hypothetical protein compare
Pf6N2E2_724 +1.2 1.2 Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family) compare
Pf6N2E2_5316 +1.2 1.6 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_4531 +1.2 2.1 hypothetical protein compare
Pf6N2E2_1083 +1.2 2.9 hypothetical protein compare
Pf6N2E2_2044 +1.1 8.1 Histidine kinase/response regulator hybrid protein compare
Pf6N2E2_953 +1.1 3.5 FIG00958851: hypothetical protein compare
Pf6N2E2_4121 +1.1 4.0 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf6N2E2_2755 +1.1 2.2 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_3841 +1.1 5.6 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3275 +1.1 3.3 FOG: TPR repeat compare
Pf6N2E2_3665 +1.1 1.1 serine/threonine protein kinase compare
Pf6N2E2_1674 +1.1 4.7 Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) compare
Pf6N2E2_3664 +1.1 6.2 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_2642 +1.1 7.3 putative patatin-like phospholipase compare
Pf6N2E2_942 +1.1 1.4 Mll6465 protein compare
Pf6N2E2_2534 +1.1 3.1 Cell division protein BolA compare
Pf6N2E2_2108 +1.0 4.4 hypothetical protein compare
Pf6N2E2_4838 +1.0 6.4 Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-) compare
Pf6N2E2_3248 +1.0 3.9 Probable transmembrane protein compare
Pf6N2E2_5078 +1.0 2.2 Carbon starvation protein A compare
Pf6N2E2_1705 +1.0 1.5 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_4554 +1.0 6.1 Thiol-disulfide isomerase and thioredoxins compare
Pf6N2E2_3027 +1.0 1.6 Baseplate assembly protein V compare
Pf6N2E2_3638 +1.0 2.4 hypothetical protein compare
Pf6N2E2_3634 +1.0 0.9 Transporter compare
Pf6N2E2_3940 +1.0 5.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2324 +1.0 3.2 Adenine phosphoribosyltransferase (EC 2.4.2.7) compare
Pf6N2E2_1626 +1.0 4.7 Putative ATP-binding component of a transport system compare
Pf6N2E2_3839 +1.0 3.5 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_4617 +1.0 7.5 Signal transduction histidine kinase CheA (EC 2.7.3.-) compare
Pf6N2E2_2312 +1.0 1.0 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_2195 +1.0 2.4 cI compare
Pf6N2E2_5176 +0.9 4.7 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_2281 +0.9 1.4 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_4810 +0.9 4.2 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_2693 +0.9 1.2 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_1444 +0.9 2.6 Transcriptional regulator, LysR family compare
Pf6N2E2_5771 +0.9 4.3 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_4618 +0.9 2.3 Chemotaxis signal transduction protein compare
Pf6N2E2_1633 +0.9 2.4 Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1) compare
Pf6N2E2_3009 +0.9 2.5 Phage tail tube protein compare
Pf6N2E2_4654 +0.9 3.3 hypothetical protein compare
Pf6N2E2_841 +0.9 3.0 hypothetical protein compare
Pf6N2E2_4107 +0.9 6.1 Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone compare
Pf6N2E2_2825 +0.9 2.5 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_3274 +0.9 3.7 Manganese superoxide dismutase (EC 1.15.1.1) compare
Pf6N2E2_4054 +0.9 1.8 Osmotically inducible protein C compare
Pf6N2E2_1445 +0.9 3.0 Glutathione S-transferase compare
Pf6N2E2_4581 +0.9 3.9 FIG015547: peptidase, M16 family compare
Pf6N2E2_5956 +0.9 3.3 FIG004453: protein YceG like compare
Pf6N2E2_5229 +0.9 3.8 Transcriptional regulator, MerR family, associated with photolyase compare
Pf6N2E2_2580 +0.9 1.8 Flagellar biosynthesis protein FliP compare
Pf6N2E2_2754 +0.9 2.3 Sigma factor RpoE negative regulatory protein RseB precursor compare
Pf6N2E2_5250 +0.9 2.9 Restriction endonuclease compare
Pf6N2E2_1548 +0.9 2.1 Lactoylglutathione lyase compare
Pf6N2E2_5175 +0.9 6.0 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_4821 +0.9 4.0 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf6N2E2_4007 +0.9 2.5 FIG00956018: hypothetical protein compare
Pf6N2E2_4405 +0.9 4.1 sensor histidine kinase compare
Pf6N2E2_6029 +0.9 1.6 hypothetical protein compare
Pf6N2E2_2579 +0.8 3.2 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_2606 +0.8 3.8 hypothetical protein compare
Pf6N2E2_4809 +0.8 6.2 COG3178: Predicted phosphotransferase related to Ser/Thr protein kinases compare
Pf6N2E2_586 +0.8 4.0 Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-) compare
Pf6N2E2_955 +0.8 1.9 FIG00953508: hypothetical protein compare
Pf6N2E2_161 +0.8 1.8 Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1) compare
Pf6N2E2_5622 +0.8 2.0 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_2842 +0.8 2.1 hypothetical protein compare
Pf6N2E2_6100 +0.8 1.7 tRNA-Val-TAC compare
Pf6N2E2_1392 +0.8 2.2 sensor histidine kinase compare
Pf6N2E2_4187 +0.8 3.0 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_692 +0.8 2.2 Glucarate dehydratase (EC 4.2.1.40) compare
Pf6N2E2_2286 +0.8 2.1 FIG00953287: hypothetical protein compare
Pf6N2E2_2363 +0.8 1.9 Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components) compare
Pf6N2E2_2519 +0.8 1.0 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_2597 +0.8 4.7 Flagellar regulatory protein FleQ compare
Pf6N2E2_4640 +0.8 1.2 hypothetical protein compare
Pf6N2E2_4454 +0.8 3.6 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_2633 +0.8 1.4 Nitroreductase family protein compare
Pf6N2E2_5709 +0.8 2.7 Protein sprT compare
Pf6N2E2_3850 +0.8 5.2 FIG074102: hypothetical protein compare
Pf6N2E2_2575 +0.8 3.5 Flagellar biosynthesis protein FlhF compare
Pf6N2E2_2971 +0.8 5.8 AmpG permease compare
Pf6N2E2_525 +0.8 3.1 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_3277 +0.8 1.5 FagA protein compare
Pf6N2E2_5713 +0.8 4.0 FIG00956341: hypothetical protein compare
Pf6N2E2_69 +0.7 3.4 Probable type IV pilus assembly FimV-related transmembrane protein compare
Pf6N2E2_328 +0.7 2.1 hypothetical protein compare
Pf6N2E2_504 +0.7 1.9 DNA-binding response regulator compare
Pf6N2E2_5384 +0.7 3.2 outer membrane protein AlgE compare
Pf6N2E2_6075 +0.7 2.4 Chromosome segregation ATPases compare
Pf6N2E2_1049 +0.7 2.5 hypothetical protein compare
Pf6N2E2_3190 +0.7 0.7 Iron-sulfur cluster regulator IscR compare
Pf6N2E2_836 +0.7 3.6 ABC-type protease/lipase transport system, ATPase and permease components compare
Pf6N2E2_578 +0.7 2.3 Mobile element protein compare
Pf6N2E2_5279 +0.7 2.4 Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C) compare
Pf6N2E2_2788 +0.7 1.3 Transcriptional regulator, AsnC family compare
Pf6N2E2_2535 +0.7 2.8 FIG00954136: hypothetical protein compare
Pf6N2E2_5044 +0.7 2.5 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_1718 +0.7 1.0 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) compare
Pf6N2E2_5597 +0.7 2.7 Electron transport complex protein RnfD compare
Pf6N2E2_1479 +0.7 2.2 Subtilisin-like serine proteases compare
Pf6N2E2_2790 +0.7 0.9 Helix-turn-helix motif compare
Pf6N2E2_5613 +0.7 1.1 FIG00953674: hypothetical protein compare
Pf6N2E2_3033 +0.7 1.1 hypothetical protein compare
Pf6N2E2_2518 +0.7 1.4 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_3870 +0.7 0.9 hypothetical protein compare
Pf6N2E2_2277 +0.7 1.8 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_958 +0.7 1.7 hypothetical protein compare
Pf6N2E2_5734 +0.7 3.1 NLP/P60 family protein compare
Pf6N2E2_5993 +0.7 1.2 FIG00957412: hypothetical protein compare
Pf6N2E2_905 +0.7 1.7 FIG00962753: hypothetical protein compare
Pf6N2E2_3072 +0.7 3.8 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_3221 +0.7 1.4 FIG00352444: hypothetical protein compare
Pf6N2E2_1111 +0.7 2.6 cell wall degradation protein compare
Pf6N2E2_5461 +0.7 2.4 Ferredoxin-dependent glutamate synthase (EC 1.4.7.1) compare
Pf6N2E2_3093 +0.7 1.8 Transcriptional regulator, TetR family compare
Pf6N2E2_3450 +0.7 3.2 protein compare
Pf6N2E2_1323 +0.7 1.1 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_4957 +0.7 2.9 Rhs family protein compare
Pf6N2E2_1860 +0.7 2.0 Ferredoxin, 2Fe-2S compare
Pf6N2E2_3541 +0.7 2.3 Putative multicopper oxidases compare
Pf6N2E2_3100 +0.7 1.4 FIG00953063: hypothetical protein compare
Pf6N2E2_5906 +0.7 1.0 Flp pilus assembly protein, pilin Flp compare
Pf6N2E2_3973 +0.7 2.4 hypothetical protein compare
Pf6N2E2_1683 +0.7 1.6 Fe2+-dicitrate sensor, membrane component compare
Pf6N2E2_945 +0.7 1.7 Transcriptional regulator, TetR family compare
Pf6N2E2_4496 +0.7 3.3 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_6040 +0.7 1.8 hypothetical protein compare
Pf6N2E2_5876 +0.7 1.7 Lipase (EC 3.1.1.3) compare
Pf6N2E2_4710 +0.6 1.0 hypothetical protein compare
Pf6N2E2_1157 +0.6 1.8 hypothetical protein possibly connected to lactam utilization and allophanate hydrolase compare
Pf6N2E2_5733 +0.6 1.7 NLP/P60 family protein compare
Pf6N2E2_3379 +0.6 4.1 Lactate-responsive regulator LldR in Enterobacteria, GntR family compare
Pf6N2E2_3872 +0.6 0.9 hypothetical protein compare
Pf6N2E2_5163 +0.6 3.7 Iron-sulfur cluster-binding protein, Rieske family compare
Pf6N2E2_1677 +0.6 1.9 Chalcone synthase (EC 2.3.1.74) compare
Pf6N2E2_6099 +0.6 0.8 tRNA-Glu-TTC compare
Pf6N2E2_759 +0.6 2.8 HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217) compare


Specific Phenotypes

For 6 genes in this experiment

For stress DEANONOate in Pseudomonas fluorescens FW300-N2E2

For stress DEANONOate across organisms