Experiment setAIT049 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Potassium chromate 0.0000015625 M

Group: stress
Media: LB + Potassium chromate (1.56e-06 M)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
By: Hans_Hualan on 2/5/2016
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 35 genes in this experiment

For stress Potassium chromate in Pseudomonas fluorescens FW300-N2E2

For stress Potassium chromate across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 7
Methionine Biosynthesis 5
DNA repair, UvrABC system 3
Lysine degradation 2
Methionine Degradation 2
Alanine biosynthesis 1
Choline and Betaine Uptake and Betaine Biosynthesis 1
Conserved gene cluster possibly involved in RNA metabolism 1
DNA repair, bacterial DinG and relatives 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
Glycine and Serine Utilization 1
Hemin transport system 1
Methionine Salvage 1
Pyruvate Alanine Serine Interconversions 1
Threonine anaerobic catabolism gene cluster 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-cysteine biosynthesis I 2 2 2
L-aspartate degradation I 1 1 1
L-cysteine degradation IV 1 1 1
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
L-alanine biosynthesis III 1 1 1
L-aspartate biosynthesis 1 1 1
thiosulfate disproportionation IV (rhodanese) 1 1 1
L-glutamate degradation II 2 2 1
atromentin biosynthesis 2 1 1
L-tyrosine degradation II 2 1 1
malate/L-aspartate shuttle pathway 2 1 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
cytidylyl molybdenum cofactor sulfurylation 2 1 1
seleno-amino acid biosynthesis (plants) 5 3 2
L-phenylalanine biosynthesis I 3 3 1
L-tyrosine biosynthesis I 3 3 1
L-phenylalanine degradation II (anaerobic) 3 2 1
L-asparagine degradation III (mammalian) 3 2 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
sulfolactate degradation III 3 1 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
(R)-cysteate degradation 3 1 1
bis(guanylyl molybdopterin) cofactor sulfurylation 3 1 1
molybdenum cofactor biosynthesis 3 1 1
5'-deoxyadenosine degradation I 3 1 1
thiazole component of thiamine diphosphate biosynthesis II 7 4 2
superpathway of L-alanine biosynthesis 4 4 1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) 4 3 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 3 1
L-tyrosine degradation III 4 2 1
L-phenylalanine degradation III 4 2 1
N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis 4 1 1
5'-deoxyadenosine degradation II 4 1 1
tRNA-uridine 2-thiolation (yeast mitochondria) 4 1 1
tRNA-uridine 2-thiolation (mammalian mitochondria) 4 1 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 2
L-tyrosine degradation I 5 5 1
trans-4-hydroxy-L-proline degradation I 5 3 1
[2Fe-2S] iron-sulfur cluster biosynthesis 10 4 2
sulfide oxidation IV (mitochondria) 5 2 1
superpathway of plastoquinol biosynthesis 5 2 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III 5 2 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II 5 2 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
phosphatidate metabolism, as a signaling molecule 5 1 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
tRNA-uridine 2-thiolation (thermophilic bacteria) 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
superpathway of thiamine diphosphate biosynthesis II 11 8 2
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 2
superpathway of L-threonine biosynthesis 6 6 1
molybdopterin biosynthesis 6 4 1
TCA cycle VIII (Chlamydia) 6 4 1
thiazole component of thiamine diphosphate biosynthesis I 6 3 1
superpathway of sulfolactate degradation 6 2 1
D-cycloserine biosynthesis 6 1 1
hydrogen sulfide biosynthesis II (mammalian) 6 1 1
coenzyme M biosynthesis II 6 1 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 2
anaerobic energy metabolism (invertebrates, cytosol) 7 4 1
diacylglycerol and triacylglycerol biosynthesis 7 3 1
L-cysteine biosynthesis VI (reverse transsulfuration) 7 3 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I 7 2 1
tRNA-uridine 2-thiolation (cytoplasmic) 8 1 1
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 5 1
superpathway of seleno-compound metabolism 19 6 2
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of thiamine diphosphate biosynthesis I 10 7 1
rosmarinic acid biosynthesis I 10 3 1
L-methionine salvage cycle III 11 5 1
tRNA-uridine 2-thiolation and selenation (bacteria) 11 4 1
L-methionine salvage cycle II (plants) 11 3 1
(S)-reticuline biosynthesis I 11 1 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
indole-3-acetate biosynthesis II 12 5 1
L-methionine salvage cycle I (bacteria and plants) 12 4 1
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of rosmarinic acid biosynthesis 14 4 1
superpathway of anaerobic energy metabolism (invertebrates) 17 8 1
type I lipoteichoic acid biosynthesis (S. aureus) 17 5 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 22 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 4 1
superpathway of chorismate metabolism 59 43 2
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1