Experiment setAH37 for Dinoroseobacter shibae DFL-12
Anaerobic growth with nitrate as electron acceptor, marine broth
Group: anaerobicMedia: marine_broth_2216 + Sodium nitrate (20 mM)
Culturing: Dino_ML3, hungate, Anaerobic, at 30 (C), shaken=200 rpm
By: Jordan on 8/22/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Specific Phenotypes
For 5 genes in this experiment
For anaerobic Sodium nitrate in Dinoroseobacter shibae DFL-12
For anaerobic Sodium nitrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biogenesis of c-type cytochromes | 1 |
| Copper homeostasis | 1 |
| Denitrification | 1 |
| Dissimilatory nitrite reductase | 1 |
| Heme biosynthesis orphans | 1 |
| Peptidoglycan Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| nitrate reduction I (denitrification) | 5 | 3 | 1 |
| nitrifier denitrification | 6 | 3 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 3 | 2 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |