Experiment set9S373 for Enterobacter sp. TBS_079

Inosine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Inosine (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 6 genes in this experiment

For carbon source Inosine in Enterobacter sp. TBS_079

For carbon source Inosine across organisms

SEED Subsystems

Subsystem	#Specific
Deoxyribose and Deoxynucleoside Catabolism	3
Adenosyl nucleosidases	1
Purine conversions	1
Xanthosine utilization (xap region)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Pentose phosphate pathway
• Pyrimidine metabolism
• Nicotinate and nicotinamide metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
purine ribonucleosides degradation	6	6	5
purine deoxyribonucleosides degradation I	4	4	3
purine deoxyribonucleosides degradation II	3	3	2
superpathway of purine deoxyribonucleosides degradation	7	7	4
adenine and adenosine salvage III	4	4	2
adenine and adenosine salvage I	2	2	1
xanthine and xanthosine salvage	2	2	1
guanine and guanosine salvage I	2	2	1
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	2
pyrimidine deoxyribonucleosides degradation	3	3	1
superpathway of guanine and guanosine salvage	3	3	1
adenine and adenosine salvage V	3	3	1
2-deoxy-α-D-ribose 1-phosphate degradation	3	3	1
purine nucleotides degradation II (aerobic)	11	9	3
guanosine nucleotides degradation III	4	3	1
inosine 5'-phosphate degradation	4	3	1
adenosine nucleotides degradation II	5	3	1
nucleoside and nucleotide degradation (archaea)	10	4	2
nucleoside and nucleotide degradation (halobacteria)	6	1	1
fluoroacetate and fluorothreonine biosynthesis	6	1	1
superpathway of purine nucleotide salvage	14	13	2
ureide biosynthesis	7	6	1
salinosporamide A biosynthesis	15	3	1
arsenic detoxification (mammals)	17	8	1