Experiment set9S341 for Enterobacter sp. TBS_079

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L-Glutamic acid monopotassium salt monohydrate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Glutamic acid monopotassium salt monohydrate (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 12 genes in this experiment

For carbon source L-Glutamic acid monopotassium salt monohydrate in Enterobacter sp. TBS_079

For carbon source L-Glutamic acid monopotassium salt monohydrate across organisms

SEED Subsystems

Subsystem #Specific
Entner-Doudoroff Pathway 1
Glutathione: Biosynthesis and gamma-glutamyl cycle 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Histidine Degradation 1
Maltose and Maltodextrin Utilization 1
Phosphate metabolism 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
RNA processing and degradation, bacterial 1
Stringent Response, (p)ppGpp metabolism 1
Utilization of glutathione as a sulphur source 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
polyphosphate metabolism 2 2 1
glutathione degradation (DUG pathway) 2 2 1
microcin B17 biosynthesis 3 1 1
tRNA processing 10 10 2
peptido-conjugates in tissue regeneration biosynthesis 17 5 3
ppGpp metabolism 6 6 1
γ-glutamyl cycle 6 5 1
leukotriene biosynthesis 6 1 1
anaerobic energy metabolism (invertebrates, cytosol) 7 5 1
Entner-Doudoroff pathway I 9 9 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 8 1
Entner-Doudoroff pathway III (semi-phosphorylative) 9 7 1
Rubisco shunt 10 9 1
glycolysis IV 10 8 1
glycolysis V (Pyrococcus) 10 7 1
glycolysis II (from fructose 6-phosphate) 11 11 1
glycolysis III (from glucose) 11 11 1
glycolysis VI (from fructose) 11 8 1
homolactic fermentation 12 11 1
glycolysis I (from glucose 6-phosphate) 13 13 1
hypoglycin biosynthesis 14 4 1
Bifidobacterium shunt 15 12 1
mixed acid fermentation 16 16 1
glycerol degradation to butanol 16 12 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 17 1
superpathway of glucose and xylose degradation 17 16 1
superpathway of anaerobic energy metabolism (invertebrates) 17 10 1
superpathway of hexitol degradation (bacteria) 18 16 1
heterolactic fermentation 18 15 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 18 1
superpathway of anaerobic sucrose degradation 19 17 1
superpathway of N-acetylneuraminate degradation 22 19 1
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle 22 18 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1