Experiment set9S303 for Enterobacter sp. TBS_079

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a-Ketoglutaric acid disodium salt hydrate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + a-Ketoglutaric acid disodium salt hydrate (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 5 genes in this experiment

For carbon source a-Ketoglutaric acid disodium salt hydrate in Enterobacter sp. TBS_079

For carbon source a-Ketoglutaric acid disodium salt hydrate across organisms

SEED Subsystems

Subsystem #Specific
Glutathione: Biosynthesis and gamma-glutamyl cycle 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Utilization of glutathione as a sulphur source 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glutathione degradation (DUG pathway) 2 2 1
peptido-conjugates in tissue regeneration biosynthesis 17 5 3
γ-glutamyl cycle 6 5 1
leukotriene biosynthesis 6 1 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 6 1
hypoglycin biosynthesis 14 4 1