Experiment set9S175 for Phocaeicola vulgatus CL09T03C04

N-Acetyl-D-Glucosamine 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + N-Acetyl-D-Glucosamine (2.5 mg/ml)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source N-Acetyl-D-Glucosamine in Phocaeicola vulgatus CL09T03C04

For carbon source N-Acetyl-D-Glucosamine across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
Peptidyl-prolyl cis-trans isomerase	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Other glycan degradation
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
chitin degradation II (Vibrio)	6	3	2
chitin degradation III (Serratia)	7	3	1
peptidoglycan recycling II	10	2	1
peptidoglycan recycling I	14	6	1