Experiment set9S162 for Phocaeicola vulgatus CL09T03C04
D-Glucose 5 mg/ml carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + D-Glucose (5 mg/ml)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 11 genes in this experiment
For carbon source D-Glucose in Phocaeicola vulgatus CL09T03C04
For carbon source D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Iron acquisition in Vibrio | 1 |
| Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria | 1 |
| Oxidative stress | 1 |
| Ton and Tol transport systems | 1 |
| Xylose utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| superoxide radicals degradation | 2 | 1 | 1 |
| (1,4)-β-D-xylan degradation | 2 | 1 | 1 |
| reactive oxygen species degradation | 4 | 3 | 1 |
| ethene biosynthesis III (microbes) | 7 | 5 | 1 |