Experiment set9S161 for Phocaeicola vulgatus CL09T03C04

D-Glucose 5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Glucose (5 mg/ml)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya Tripathi on 3/20/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 12 genes in this experiment

For carbon source D-Glucose in Phocaeicola vulgatus CL09T03C04

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	1
Iron acquisition in Vibrio	1
L-Arabinose utilization	1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Other glycan degradation
• Aminosugars metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
chitin degradation II (Vibrio)	6	3	2
chitin degradation III (Serratia)	7	3	1
peptidoglycan recycling II	10	2	1
peptidoglycan recycling I	14	6	1