Experiment set9IT090 for Ralstonia sp. UNC404CL21Col

Lauric acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Lauric acid (10 mM)
Culturing: CL21_ML4_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 3.2 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 27 genes in this experiment

For carbon source Lauric acid in Ralstonia sp. UNC404CL21Col

For carbon source Lauric acid across organisms

SEED Subsystems

Subsystem	#Specific
Ton and Tol transport systems	3
Multidrug Resistance Efflux Pumps	2
Peptidoglycan Biosynthesis	2
Branched-Chain Amino Acid Biosynthesis	1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more.	1
Cobalt-zinc-cadmium resistance	1
Folate Biosynthesis	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Iron acquisition in Vibrio	1
Lipid A modifications	1
Pyruvate Alanine Serine Interconversions	1
Respiratory dehydrogenases 1	1
Serine-glyoxylate cycle	1
Transport of Iron	1
ZZ gjo need homes	1
cAMP signaling in bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Folate biosynthesis
• Biosynthesis of phenylpropanoids
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Glycine, serine and threonine metabolism
• Phenylalanine metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Peptidoglycan biosynthesis
• Glyoxylate and dicarboxylate metabolism
• Diterpenoid biosynthesis
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
4-aminobenzoate biosynthesis I	2	2	2
L-glutamine degradation I	1	1	1
L-alanine degradation I	2	2	1
L-glutamate biosynthesis I	2	2	1
4-aminobenzoate biosynthesis II	2	1	1
ammonia assimilation cycle III	3	3	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	3	1
superpathway of tetrahydrofolate biosynthesis	10	9	2
superpathway of candicidin biosynthesis	11	3	2
glyoxylate cycle	6	6	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	11	2
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
L-glutamate and L-glutamine biosynthesis	7	7	1
L-citrulline biosynthesis	8	7	1
chloramphenicol biosynthesis	9	1	1
superpathway of glyoxylate bypass and TCA	12	10	1
superpathway of L-citrulline metabolism	12	9	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	22	1
superpathway of chorismate metabolism	59	44	2