Experiment set9IT090 for Bacteroides thetaiotaomicron VPI-5482

BHIS with Sulfaquinoxaline sodium salt 0.1 mM

Group: stress
Media: BHIS + Sulfaquinoxaline sodium salt (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 10 genes in this experiment

For stress Sulfaquinoxaline sodium salt in Bacteroides thetaiotaomicron VPI-5482

For stress Sulfaquinoxaline sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
Adenosyl nucleosidases	1
Butanol Biosynthesis	1
Deoxyribose and Deoxynucleoside Catabolism	1
Fermentations: Mixed acid	1
Folate Biosynthesis	1
Glycine and Serine Utilization	1
Methionine Biosynthesis	1
Methionine Degradation	1
One-carbon metabolism by tetrahydropterines	1
Purine Utilization	1
Purine conversions	1
Pyruvate Alanine Serine Interconversions	1
Serine-glyoxylate cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Purine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Cysteine metabolism
• Selenoamino acid metabolism
• Pyruvate metabolism
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Butanoate metabolism
• One carbon pool by folate
• Nicotinate and nicotinamide metabolism
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-serine degradation	3	3	3
purine deoxyribonucleosides degradation I	4	3	3
formate assimilation into 5,10-methylenetetrahydrofolate	3	3	2
L-tryptophan degradation II (via pyruvate)	3	3	2
purine ribonucleosides degradation	6	4	4
L-cysteine degradation II	3	2	2
D-serine degradation	3	2	2
purine deoxyribonucleosides degradation II	3	2	2
pyrimidine ribonucleosides degradation	2	2	1
reductive monocarboxylic acid cycle	2	2	1
xanthine and xanthosine salvage	2	2	1
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate	2	2	1
adenine and adenosine salvage I	2	2	1
guanine and guanosine salvage I	2	2	1
adenine and adenosine salvage III	4	3	2
glycine betaine degradation III	7	4	3
superpathway of purine deoxyribonucleosides degradation	7	4	3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
glycine betaine degradation I	8	4	3
pyruvate fermentation to acetate IV	3	3	1
L-methionine degradation I (to L-homocysteine)	3	2	1
superpathway of guanine and guanosine salvage	3	2	1
pyruvate fermentation to ethanol I	3	2	1
pyrimidine deoxyribonucleosides degradation	3	2	1
S-adenosyl-L-methionine salvage II	3	2	1
glycine degradation	3	2	1
L-methionine biosynthesis II	6	3	2
adenine and adenosine salvage V	3	1	1
nucleoside and nucleotide degradation (archaea)	10	4	3
purine nucleotides degradation II (aerobic)	11	7	3
formaldehyde oxidation VII (THF pathway)	4	4	1
inosine 5'-phosphate degradation	4	3	1
L-mimosine degradation	8	4	2
guanosine nucleotides degradation III	4	2	1
glutathione-mediated detoxification I	8	2	2
reductive glycine pathway of autotrophic CO2 fixation	9	6	2
folate polyglutamylation	5	5	1
superpathway of pyrimidine ribonucleosides degradation	5	2	1
adenosine nucleotides degradation II	5	2	1
superpathway of L-cysteine biosynthesis (mammalian)	5	2	1
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)	10	3	2
folate transformations II (plants)	11	8	2
L-histidine degradation III	6	6	1
L-threonine degradation I	6	5	1
purine nucleobases degradation II (anaerobic)	24	12	4
nucleoside and nucleotide degradation (halobacteria)	6	3	1
superpathway of pyrimidine deoxyribonucleosides degradation	6	3	1
fluoroacetate and fluorothreonine biosynthesis	6	1	1
folate transformations I	13	8	2
superpathway of purine nucleotide salvage	14	13	2
ureide biosynthesis	7	3	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	12	2
folate transformations III (E. coli)	9	7	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	7	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
(S)-lactate fermentation to propanoate, acetate and hydrogen	13	10	1
purine nucleobases degradation I (anaerobic)	15	5	1
salinosporamide A biosynthesis	15	4	1
mixed acid fermentation	16	12	1
superpathway of L-methionine salvage and degradation	16	8	1
arsenic detoxification (mammals)	17	7	1
superpathway of L-threonine metabolism	18	13	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	14	1
superpathway of N-acetylneuraminate degradation	22	16	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	2