Experiment set9IT088 for Ralstonia sp. UNC404CL21Col

Heptanoic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Heptanoic acid (10 mM)
Culturing: CL21_ML4_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.5 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 16 genes in this experiment

For carbon source Heptanoic acid in Ralstonia sp. UNC404CL21Col

For carbon source Heptanoic acid across organisms

SEED Subsystems

Subsystem	#Specific
Biotin biosynthesis	1
Glycine and Serine Utilization	1
Glycine cleavage system	1
Phosphate metabolism	1
Photorespiration (oxidative C2 cycle)	1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate	1
Redox-dependent regulation of nucleus processes	1
Ton and Tol transport systems	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• Glutathione metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Sphingolipid metabolism
• 1,4-Dichlorobenzene degradation
• Nicotinate and nicotinamide metabolism
• Biotin metabolism
• Caprolactam degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cis-cyclopropane fatty acid (CFA) biosynthesis	1	1	1
8-amino-7-oxononanoate biosynthesis II	2	1	1
sterculate biosynthesis	2	1	1
8-amino-7-oxononanoate biosynthesis III	2	1	1
glycine cleavage	3	3	1
glycine biosynthesis II	3	3	1
8-amino-7-oxononanoate biosynthesis IV	5	5	1
biotin biosynthesis II	6	5	1
8-amino-7-oxononanoate biosynthesis I	11	9	1
biotin biosynthesis I	15	13	1
mycolate biosynthesis	205	25	3
superpathway of mycolate biosynthesis	239	26	3