Experiment set9IT080 for Ralstonia sp. UNC404CL21Col

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Dodecandioic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Dodecandioic acid (10 mM)
Culturing: CL21_ML4_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 3.3 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 7 genes in this experiment

For carbon source Dodecandioic acid in Ralstonia sp. UNC404CL21Col

For carbon source Dodecandioic acid across organisms

SEED Subsystems

Subsystem #Specific
Biotin biosynthesis 1
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Folate Biosynthesis 1
Terminal cytochrome O ubiquinol oxidase 1
Terminal cytochrome oxidases 1
Universal stress protein family 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
4-aminobenzoate biosynthesis I 2 2 2
L-glutamine degradation I 1 1 1
succinate to cytochrome bo oxidase electron transfer 2 2 1
L-glutamate biosynthesis I 2 2 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
pyruvate to cytochrome bo oxidase electron transfer 2 2 1
glycerol-3-phosphate to cytochrome bo oxidase electron transfer 2 2 1
proline to cytochrome bo oxidase electron transfer 2 1 1
4-aminobenzoate biosynthesis II 2 1 1
8-amino-7-oxononanoate biosynthesis III 2 1 1
NADH to cytochrome bo oxidase electron transfer II 2 1 1
8-amino-7-oxononanoate biosynthesis II 2 1 1
D-lactate to cytochrome bo oxidase electron transfer 2 1 1
ammonia assimilation cycle III 3 3 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
8-amino-7-oxononanoate biosynthesis IV 5 5 1
superpathway of tetrahydrofolate biosynthesis 10 9 2
superpathway of candicidin biosynthesis 11 3 2
superpathway of tetrahydrofolate biosynthesis and salvage 12 11 2
biotin biosynthesis II 6 5 1
L-glutamate and L-glutamine biosynthesis 7 7 1
L-citrulline biosynthesis 8 7 1
chloramphenicol biosynthesis 9 1 1
8-amino-7-oxononanoate biosynthesis I 11 9 1
superpathway of L-citrulline metabolism 12 9 1
biotin biosynthesis I 15 13 1
superpathway of chorismate metabolism 59 44 2