Experiment set9IT079 for Bacteroides thetaiotaomicron VPI-5482

BHIS with Zinc Pyrithione 0.025 mM

Group: stress
Media: BHIS + Zinc Pyrithione (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 7 genes in this experiment

For stress Zinc Pyrithione in Bacteroides thetaiotaomicron VPI-5482

For stress Zinc Pyrithione across organisms

SEED Subsystems

Subsystem	#Specific
Oxidative stress	1
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Histidine metabolism
• beta-Alanine metabolism
• Urea cycle and metabolism of amino groups
• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Glutathione metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glutathione degradation (DUG pathway)	2	1	1
muropeptide degradation	4	2	1
γ-glutamyl cycle	6	1	1