Culturing: Smeli_ML6_JBEI, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| L-aspartate biosynthesis | 1 | 1 | 1 |
| L-aspartate degradation I | 1 | 1 | 1 |
| 3-(4-hydroxyphenyl)pyruvate biosynthesis | 1 | 1 | 1 |
| pentose phosphate pathway (partial) | 3 | 3 | 2 |
| acetoacetate degradation (to acetyl CoA) | 2 | 2 | 1 |
| glycolate and glyoxylate degradation II | 2 | 2 | 1 |
| malate/L-aspartate shuttle pathway | 2 | 2 | 1 |
| pentose phosphate pathway (non-oxidative branch) II | 6 | 5 | 3 |
| L-glutamate degradation II | 2 | 1 | 1 |
| L-tryptophan degradation IV (via indole-3-lactate) | 2 | 1 | 1 |
| atromentin biosynthesis | 2 | 1 | 1 |
| L-tyrosine degradation II | 2 | 1 | 1 |
| sedoheptulose bisphosphate bypass | 2 | 1 | 1 |
| pentose phosphate pathway (non-oxidative branch) I | 5 | 5 | 2 |
| 5,6-dehydrokavain biosynthesis (engineered) | 10 | 6 | 4 |
| Calvin-Benson-Bassham cycle | 13 | 12 | 5 |
| polyhydroxybutanoate biosynthesis | 3 | 3 | 1 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| L-tyrosine biosynthesis I | 3 | 3 | 1 |
| L-phenylalanine biosynthesis I | 3 | 3 | 1 |
| photorespiration III | 9 | 8 | 3 |
| formaldehyde assimilation II (assimilatory RuMP Cycle) | 9 | 7 | 3 |
| L-phenylalanine degradation II (anaerobic) | 3 | 2 | 1 |
| 5'-deoxyadenosine degradation I | 3 | 2 | 1 |
| ketolysis | 3 | 2 | 1 |
| glycolate and glyoxylate degradation III | 3 | 1 | 1 |
| indole-3-acetate biosynthesis VI (bacteria) | 3 | 1 | 1 |
| sulfolactate degradation III | 3 | 1 | 1 |
| (R)-cysteate degradation | 3 | 1 | 1 |
| L-tyrosine degradation IV (to 4-methylphenol) | 3 | 1 | 1 |
| L-asparagine degradation III (mammalian) | 3 | 1 | 1 |
| Rubisco shunt | 10 | 10 | 3 |
| photorespiration II | 10 | 8 | 3 |
| oxygenic photosynthesis | 17 | 13 | 5 |
| pentose phosphate pathway | 8 | 8 | 2 |
| 5'-deoxyadenosine degradation II | 4 | 4 | 1 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | 12 | 11 | 3 |
| glycolate and glyoxylate degradation I | 4 | 3 | 1 |
| (2S)-ethylmalonyl-CoA biosynthesis | 4 | 3 | 1 |
| L-tyrosine degradation III | 4 | 2 | 1 |
| L-phenylalanine degradation III | 4 | 2 | 1 |
| L-tryptophan degradation VIII (to tryptophol) | 4 | 1 | 1 |
| superpathway of L-aspartate and L-asparagine biosynthesis | 4 | 1 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 22 | 6 |
| oleate β-oxidation | 35 | 29 | 8 |
| photorespiration I | 9 | 8 | 2 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 21 | 6 |
| L-lysine degradation V | 9 | 5 | 2 |
| valproate β-oxidation | 9 | 5 | 2 |
| L-lysine degradation II (L-pipecolate pathway) | 9 | 4 | 2 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 9 | 3 |
| (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) | 5 | 5 | 1 |
| L-tyrosine degradation I | 5 | 5 | 1 |
| ethene biosynthesis V (engineered) | 25 | 19 | 5 |
| trans-4-hydroxy-L-proline degradation I | 5 | 3 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation III | 5 | 3 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 3 | 1 |
| ketogenesis | 5 | 3 | 1 |
| 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) | 10 | 4 | 2 |
| pyruvate fermentation to acetone | 5 | 2 | 1 |
| L-lysine degradation XI | 5 | 2 | 1 |
| superpathway of plastoquinol biosynthesis | 5 | 2 | 1 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 2 | 1 |
| sucrose degradation V (sucrose α-glucosidase) | 5 | 2 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation II | 5 | 2 | 1 |
| L-tryptophan degradation XIII (reductive Stickland reaction) | 5 | 1 | 1 |
| L-tyrosine degradation V (reductive Stickland reaction) | 5 | 1 | 1 |
| L-phenylalanine degradation VI (reductive Stickland reaction) | 5 | 1 | 1 |
| fatty acid β-oxidation VII (yeast peroxisome) | 5 | 1 | 1 |
| 4-hydroxybenzoate biosynthesis I (eukaryotes) | 5 | 1 | 1 |
| isopropanol biosynthesis (engineered) | 5 | 1 | 1 |
| ethylbenzene degradation (anaerobic) | 5 | 1 | 1 |
| C4 photosynthetic carbon assimilation cycle, NAD-ME type | 11 | 7 | 2 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| superpathway of L-threonine biosynthesis | 6 | 6 | 1 |
| glyoxylate cycle | 6 | 6 | 1 |
| TCA cycle VIII (Chlamydia) | 6 | 6 | 1 |
| fatty acid salvage | 6 | 5 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| L-isoleucine degradation I | 6 | 4 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 1 |
| superpathway of sulfolactate degradation | 6 | 3 | 1 |
| 4-ethylphenol degradation (anaerobic) | 6 | 2 | 1 |
| coenzyme M biosynthesis II | 6 | 1 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) | 6 | 1 | 1 |
| hydrogen sulfide biosynthesis II (mammalian) | 6 | 1 | 1 |
| jasmonic acid biosynthesis | 19 | 4 | 3 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 2 |
| acetyl-CoA fermentation to butanoate | 7 | 6 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 5 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 1 |
| superpathway of glycol metabolism and degradation | 7 | 5 | 1 |
| C4 photosynthetic carbon assimilation cycle, PEPCK type | 14 | 8 | 2 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 3 | 1 |
| mevalonate pathway II (haloarchaea) | 7 | 1 | 1 |
| mevalonate pathway I (eukaryotes and bacteria) | 7 | 1 | 1 |
| S-methyl-5-thio-α-D-ribose 1-phosphate degradation I | 7 | 1 | 1 |
| pyruvate fermentation to butanol I | 8 | 4 | 1 |
| 2-deoxy-D-ribose degradation II | 8 | 3 | 1 |
| 2-methylpropene degradation | 8 | 2 | 1 |
| isoprene biosynthesis II (engineered) | 8 | 1 | 1 |
| mevalonate pathway IV (archaea) | 8 | 1 | 1 |
| mevalonate pathway III (Thermoplasma) | 8 | 1 | 1 |
| androstenedione degradation I (aerobic) | 25 | 6 | 3 |
| superpathway of glucose and xylose degradation | 17 | 15 | 2 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 2 |
| sucrose biosynthesis I (from photosynthesis) | 9 | 7 | 1 |
| superpathway of L-methionine biosynthesis (transsulfuration) | 9 | 7 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| 1,3-propanediol biosynthesis (engineered) | 9 | 5 | 1 |
| L-phenylalanine degradation IV (mammalian, via side chain) | 9 | 4 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| 4-oxopentanoate degradation | 9 | 2 | 1 |
| L-lysine biosynthesis IV | 9 | 2 | 1 |
| superpathway of testosterone and androsterone degradation | 28 | 6 | 3 |
| superpathway of L-tyrosine biosynthesis | 10 | 10 | 1 |
| superpathway of L-phenylalanine biosynthesis | 10 | 10 | 1 |
| glycolysis IV | 10 | 8 | 1 |
| glycolysis V (Pyrococcus) | 10 | 6 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 6 | 1 |
| 3-phenylpropanoate degradation | 10 | 4 | 1 |
| nucleoside and nucleotide degradation (archaea) | 10 | 4 | 1 |
| superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) | 10 | 4 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 1 |
| rosmarinic acid biosynthesis I | 10 | 2 | 1 |
| L-lysine biosynthesis V | 10 | 2 | 1 |
| methyl tert-butyl ether degradation | 10 | 2 | 1 |
| superpathway of cholesterol degradation I (cholesterol oxidase) | 42 | 8 | 4 |
| glycolysis III (from glucose) | 11 | 10 | 1 |
| glycolysis II (from fructose 6-phosphate) | 11 | 9 | 1 |
| glycolysis VI (from fructose) | 11 | 7 | 1 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 1 |
| ethylmalonyl-CoA pathway | 11 | 4 | 1 |
| L-methionine salvage cycle II (plants) | 11 | 4 | 1 |
| (S)-reticuline biosynthesis I | 11 | 3 | 1 |
| L-methionine salvage cycle III | 11 | 2 | 1 |
| superpathway of cholesterol degradation II (cholesterol dehydrogenase) | 47 | 8 | 4 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 11 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
| homolactic fermentation | 12 | 10 | 1 |
| gluconeogenesis III | 12 | 9 | 1 |
| indole-3-acetate biosynthesis II | 12 | 5 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 1 |
| L-methionine salvage cycle I (bacteria and plants) | 12 | 4 | 1 |
| 10-cis-heptadecenoyl-CoA degradation (yeast) | 12 | 2 | 1 |
| 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) | 12 | 2 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| gluconeogenesis I | 13 | 12 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 22 | 2 |
| glycolysis I (from glucose 6-phosphate) | 13 | 10 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 6 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 2 | 1 |
| androstenedione degradation II (anaerobic) | 27 | 4 | 2 |
| superpathway of rosmarinic acid biosynthesis | 14 | 3 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 2 | 1 |
| superpathway of L-lysine degradation | 43 | 15 | 3 |
| Bifidobacterium shunt | 15 | 14 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 3 | 1 |
| glycerol degradation to butanol | 16 | 12 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 3 | 1 |
| superpathway of glycolysis and the Entner-Doudoroff pathway | 17 | 14 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 12 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 8 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 3 | 1 |
| cholesterol degradation to androstenedione I (cholesterol oxidase) | 17 | 2 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 16 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 13 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 10 | 1 |
| gluconeogenesis II (Methanobacterium thermoautotrophicum) | 18 | 7 | 1 |
| toluene degradation VI (anaerobic) | 18 | 3 | 1 |
| sitosterol degradation to androstenedione | 18 | 1 | 1 |
| Methanobacterium thermoautotrophicum biosynthetic metabolism | 56 | 20 | 3 |
| superpathway of anaerobic sucrose degradation | 19 | 14 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 12 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 19 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 16 | 1 |
| cholesterol degradation to androstenedione II (cholesterol dehydrogenase) | 22 | 2 | 1 |
| superpathway of cholesterol degradation III (oxidase) | 49 | 4 | 2 |
| aspartate superpathway | 25 | 23 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| superpathway of ergosterol biosynthesis I | 26 | 3 | 1 |
| anaerobic aromatic compound degradation (Thauera aromatica) | 27 | 3 | 1 |
| superpathway of chorismate metabolism | 59 | 38 | 2 |
| superpathway of cholesterol biosynthesis | 38 | 3 | 1 |