Experiment set9IT073 for Sinorhizobium meliloti 1021

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Potassium acetate carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Potassium acetate (10 mM)
Culturing: Smeli_ML6_JBEI, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Catharine Adams on 11/8/20
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 9 genes in this experiment

For carbon source Potassium acetate in Sinorhizobium meliloti 1021

For carbon source Potassium acetate across organisms

SEED Subsystems

Subsystem #Specific
Dihydroxyacetone kinases 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Histidine Degradation 1
Ketoisovalerate oxidoreductase 1
Peptidoglycan Biosynthesis 1
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
acetate conversion to acetyl-CoA 1 1 1
acetate and ATP formation from acetyl-CoA III 1 1 1
glycerol degradation II 2 2 1
ethanol degradation II 3 3 1
ethanol degradation IV 3 3 1
superpathway of acetate utilization and formation 3 3 1
L-isoleucine biosynthesis V 3 2 1
ethanol degradation III 3 2 1
2-chloroacrylate degradation I 3 2 1
chitin deacetylation 4 3 1
superpathway of glycerol degradation to 1,3-propanediol 4 2 1
L-histidine degradation II 5 5 1
2-methylcitrate cycle I 5 1 1
glyoxylate cycle 6 6 1
β-alanine biosynthesis II 6 5 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of bitter acids biosynthesis 18 3 3
lupulone and humulone biosynthesis 6 1 1
2-methylcitrate cycle II 6 1 1
colupulone and cohumulone biosynthesis 6 1 1
adlupulone and adhumulone biosynthesis 6 1 1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) 8 8 1
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) 8 7 1
reductive glycine pathway of autotrophic CO2 fixation 9 6 1
cis-geranyl-CoA degradation 9 2 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 11 1
superpathway of glyoxylate bypass and TCA 12 11 1
peptidoglycan biosynthesis I (meso-diaminopimelate containing) 12 11 1
superpathway of glyoxylate cycle and fatty acid degradation 14 12 1
peptidoglycan biosynthesis III (mycobacteria) 15 11 1
glycerol degradation to butanol 16 12 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
peptidoglycan biosynthesis V (β-lactam resistance) 17 11 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 22 1