Experiment set9IT073 for Ralstonia sp. UNC404CL21Col
Lauric acid carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + Lauric acid (10 mM)
Culturing: CL21_ML4_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 1.4 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 7 genes in this experiment
For carbon source Lauric acid in Ralstonia sp. UNC404CL21Col
For carbon source Lauric acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Malonate decarboxylase | 7 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| malonate degradation I (biotin-independent) | 3 | 2 | 2 |
| fatty acid biosynthesis initiation (type II) | 3 | 3 | 1 |
| fatty acid biosynthesis initiation (mitochondria) | 4 | 2 | 1 |
| malonate degradation II (biotin-dependent) | 4 | 1 | 1 |
| fatty acid biosynthesis initiation (plant mitochondria) | 4 | 1 | 1 |
| superpathway of fatty acid biosynthesis initiation | 5 | 4 | 1 |
| pederin biosynthesis | 14 | 2 | 2 |
| bryostatin biosynthesis | 19 | 2 | 2 |
| mycobactin biosynthesis | 11 | 3 | 1 |
| superpathway of fatty acid biosynthesis I (E. coli) | 16 | 14 | 1 |
| mupirocin biosynthesis | 26 | 1 | 1 |
| corallopyronin A biosynthesis | 30 | 2 | 1 |
| superpathway of fatty acid biosynthesis II (plant) | 43 | 38 | 1 |
| superpathway of fatty acids biosynthesis (E. coli) | 53 | 50 | 1 |