Experiment set9IT066 for Bacteroides thetaiotaomicron VPI-5482

Group: stress
Media: BHIS + Sulfamonomethoxine (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Subsystem	#Specific
Folate Biosynthesis	1
Methionine Biosynthesis	1
Methionine Degradation	1
One-carbon metabolism by tetrahydropterines	1
Purine Utilization	1
Serine-glyoxylate cycle	1

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
formate assimilation into 5,10-methylenetetrahydrofolate	3	3	2
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate	2	2	1
S-adenosyl-L-methionine salvage II	3	2	1
L-methionine degradation I (to L-homocysteine)	3	2	1
formaldehyde oxidation VII (THF pathway)	4	4	1
reductive glycine pathway of autotrophic CO₂ fixation	9	6	2
folate polyglutamylation	5	5	1
superpathway of L-cysteine biosynthesis (mammalian)	5	2	1
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)	10	3	2
folate transformations II (plants)	11	8	2
L-histidine degradation III	6	6	1
folate transformations I	13	8	2
folate transformations III (E. coli)	9	7	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
purine nucleobases degradation I (anaerobic)	15	5	1
superpathway of L-methionine salvage and degradation	16	8	1
purine nucleobases degradation II (anaerobic)	24	12	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	20	2