Experiment set9IT059 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with Ciprofloxacin Hydrochloride 0.025 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Ciprofloxacin Hydrochloride (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For stress Ciprofloxacin Hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Ciprofloxacin Hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.