Experiment set9IT050 for Pseudomonas syringae pv. syringae B728a

KB with Chaulmoogric acid 10 mM

Group: stress
Media: KB + Chaulmoogric acid (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/23/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 1 genes in this experiment

For stress Chaulmoogric acid in Pseudomonas syringae pv. syringae B728a

For stress Chaulmoogric acid across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism
• Benzoate degradation via CoA ligation
• Limonene and pinene degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
oleate β-oxidation (thioesterase-dependent, yeast)	2	2	1
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered)	5	4	2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	19	2
oleate β-oxidation	35	30	1