Experiment set9IT045 for Klebsiella michiganensis M5al
RCH2_defined_Glucose_noNitrogen in Eppendorf tube
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen, pH=7
Culturing: Koxy_ML2, Eppendorf tube, Aerobic, at 30 (C), shaken=0 rpm
By: Trenton on 6-Mar-18
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 6 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Nitrogen fixation | 5 |
| Methionine Degradation | 1 |
| Pyruvate:ferredoxin oxidoreductase | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pyruvate metabolism
- Glycolysis / Gluconeogenesis
- Citrate cycle (TCA cycle)
- Lysine biosynthesis
- Trinitrotoluene degradation
- Propanoate metabolism
- Butanoate metabolism
- Reductive carboxylate cycle (CO2 fixation)
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: