Experiment set9IT039 for Bacteroides thetaiotaomicron VPI-5482

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BHIS with Chlorpromazine hydrochloride 0.1 mM

Group: stress
Media: BHIS + Chlorpromazine hydrochloride (0.1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 5 genes in this experiment

For stress Chlorpromazine hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Chlorpromazine hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
Acetyl-CoA fermentation to Butyrate 1
Carboxysome 1
Isoleucine degradation 1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1
Respiratory dehydrogenases 1 1
Valine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
NADH to cytochrome bo oxidase electron transfer I 2 1 1
NADH to cytochrome bd oxidase electron transfer I 2 1 1
aerobic respiration III (alternative oxidase pathway) 3 2 1
aerobic respiration I (cytochrome c) 4 2 1
(S)-propane-1,2-diol degradation 5 3 1
L-threonine degradation I 6 5 1
NAD(P)/NADPH interconversion 6 2 1
Fe(II) oxidation 6 1 1
pyruvate fermentation to butanoate 7 3 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
L-glutamate degradation VII (to butanoate) 12 4 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 6 1
superpathway of L-threonine metabolism 18 13 1