Experiment set9IT037 for Paraburkholderia bryophila 376MFSha3.1

Bu1_100

Group: phage
Media: LB + CaCl2, MgSO4 (5mM ), pH=7
Culturing: Burk376_ML3, 48-well_plate, Aerobic, at 30C (C), shaken=700 rpm
By: MM on 5/20/2021
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 12 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Choline and Betaine Uptake and Betaine Biosynthesis	1
DNA repair, UvrABC system	1
Fermentations: Mixed acid	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Heat shock dnaK gene cluster extended	1
Peptidoglycan Biosynthesis	1
Protein chaperones	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Tyrosine metabolism
• Nucleotide sugars metabolism
• Peptidoglycan biosynthesis
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Retinol metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
acetaldehyde biosynthesis I	1	1	1
ethanol degradation I	2	2	1
pyruvate fermentation to ethanol II	2	1	1
glycine betaine biosynthesis III (plants)	3	3	1
ethanol degradation II	3	3	1
pyruvate fermentation to ethanol III	3	2	1
L-valine degradation II	3	2	1
L-isoleucine degradation II	3	2	1
L-leucine degradation III	3	2	1
pyruvate fermentation to ethanol I	3	2	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	1	1
L-methionine degradation III	3	1	1
phytol degradation	4	3	1
L-phenylalanine degradation III	4	2	1
L-tyrosine degradation III	4	2	1
cytidine-5'-diphosphate-glycerol biosynthesis	4	1	1
salidroside biosynthesis	4	1	1
ethanolamine utilization	5	5	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
acetylene degradation (anaerobic)	5	4	1
(S)-propane-1,2-diol degradation	5	3	1
alcaligin biosynthesis	5	1	1
phenylethanol biosynthesis	5	1	1
peptidoglycan maturation (meso-diaminopimelate containing)	12	4	2
noradrenaline and adrenaline degradation	13	4	2
3-methylbutanol biosynthesis (engineered)	7	6	1
serotonin degradation	7	3	1
butanol and isobutanol biosynthesis (engineered)	8	4	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	1	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	6	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	7	1
L-tryptophan degradation V (side chain pathway)	13	1	1
mixed acid fermentation	16	13	1
peptidoglycan biosynthesis IV (Enterococcus faecium)	17	12	1
peptidoglycan biosynthesis II (staphylococci)	17	12	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	10	1
heterolactic fermentation	18	16	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	15	1
superpathway of anaerobic sucrose degradation	19	15	1
superpathway of N-acetylneuraminate degradation	22	16	1