Experiment set9IT036 for Bacteroides thetaiotaomicron VPI-5482

Compare to:

Varel_Bryant_medium_Glucose with Econazole nitrate salt 0.025 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Econazole nitrate salt (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For stress Econazole nitrate salt in Bacteroides thetaiotaomicron VPI-5482

For stress Econazole nitrate salt across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Ton and Tol transport systems 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis I 2 2 2
L-glutamine degradation II 1 1 1
L-glutamine degradation I 1 1 1
ammonia assimilation cycle III 3 3 2
NAD salvage pathway IV (from nicotinamide riboside) 2 1 1
L-glutamate and L-glutamine biosynthesis 7 5 2
glutaminyl-tRNAgln biosynthesis via transamidation 4 1 1
L-asparagine biosynthesis III (tRNA-dependent) 4 1 1
L-citrulline biosynthesis 8 5 1
superpathway of L-citrulline metabolism 12 8 1
superpathway of NAD biosynthesis in eukaryotes 14 6 1