Experiment set9IT031 for Cupriavidus basilensis FW507-4G11
Defined media with exometabolites; 5% oxygen; 5 mM nitrate
Group: oxygen_nitrateMedia: SGW_Northen_Exometabolite_mix_1 + Sodium nitrate (5 mM), pH=7
Culturing: cupriavidus_4G11_ML11_FieldsLab, tube, Aerobic, at 15 (C), shaken=10 rpm
By: Katie Davis on 08/18/2019
Media components: 0.6 g/L Potassium phosphate monobasic, 0.15 g/L Ammonium chloride, Northen_Exometabolite_mix_1 (880.5 uM D-Mannitol, 824.8 uM D-Glucose, 806.5 uM D-Fructose, 561.8 uM D-Trehalose dihydrate, 207.6 uM D-Arabinose, 165.4 uM D-Maltose monohydrate, 105.2 uM Arabitol, 60 uM L-Leucine, 60 uM L-Isoleucine, 40 uM Glycine, 40 uM L-Arginine, 40 uM L-Proline, 40 uM L-Threonine, 40 uM L-Lysine, 40 uM L-Phenylalanine, 39.4 uM D-Mannose, 22.5 uM Uridine, 20 uM Carnitine Hydrochloride, 20 uM L-Asparagine, 20 uM L-Serine, 20 uM L-Valine, 20 uM L-tyrosine, 20 uM L-Tryptophan, 20 uM L-Methionine, 20 uM L-Glutamic acid monopotassium salt monohydrate, 20 uM L-Citrulline, 20 uM L-Aspartic Acid, 20 uM L-Alanine, 20 uM L-Histidine, 20 uM L-Glutamine, 5.9 uM 4-Guanidinobutyric acid, 4.2 uM Hypoxanthine, 3.2 uM Uracil, 3.2 uM Thiamine HCl, 3.2 uM Guanine, 3.2 uM Cytosine, 3.2 uM Adenine hydrochloride hydrate, 2.6 uM Thymidine, 2.6 uM spermidine, 2.6 uM Orotic acid, 2.6 uM Inosine, 2.6 uM 2'-Deoxyinosine, 2.6 uM Cytidine, 2.6 uM Choline chloride, 2.6 uM Adenosine, 2.6 uM 2'-Deoxyuridine), Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
None in this experiment
For Cupriavidus basilensis FW507-4G11 in oxygen_nitrate experiments