Experiment set9IT029 for Synechococcus elongatus PCC 7942

Compare to:

12:12 LD cycle bioreactor

Group: circadian
Media: BG11, pH=7.5
Culturing: SynE_ML6.2, phenometrics bioreactor, Aerobic, at 30 (C), shaken=none (air bubbled)
Growth: about 6.5 generations
By: Ben Rubin on 10/5/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 3 genes in this experiment

SEED Subsystems

Subsystem #Specific
Alginate metabolism 1
Mannose Metabolism 1
Serine-glyoxylate cycle 1
Succinate dehydrogenase 1
TCA Cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
GDP-mannose biosynthesis 4 4 2
succinate to cytochrome bo oxidase electron transfer 2 1 1
succinate to cytochrome bo quinol oxidase (cyanobacteria, cytoplamic membrane) 2 1 1
succinate to cytochrome bd oxidase electron transfer 2 1 1
succinate to cytochrome c oxidase via plastocyanin (thylakoid membrane) 3 3 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation 3 2 1
succinate to chytochrome c oxidase via cytochrome c6 (thylakoid membrane) 3 2 1
aerobic respiration I (cytochrome c) 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 2 1
colanic acid building blocks biosynthesis 11 10 2
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis 14 10 2
β-(1,4)-mannan degradation 7 2 1
TCA cycle III (animals) 10 7 1