Experiment set9IT026 for Pseudomonas syringae pv. syringae B728a

KB with Plumbagin 10 mM

Group: stress
Media: KB + Plumbagin (10 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/23/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 14 genes in this experiment

For stress Plumbagin in Pseudomonas syringae pv. syringae B728a

For stress Plumbagin across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
Arginine and Ornithine Degradation	1
Biotin biosynthesis	1
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Mannose Metabolism	1
Pentose phosphate pathway	1
n-Phenylalkanoic acid degradation	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Fructose and mannose metabolism
• Fatty acid metabolism
• Pyrimidine metabolism
• Glutamate metabolism
• Arginine and proline metabolism
• Glutathione metabolism
• Nucleotide sugars metabolism
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
long-chain fatty acid activation	1	1	1
L-glutamate degradation I	1	1	1
D-mannose degradation I	2	1	1
linoleate biosynthesis II (animals)	2	1	1
D-mannose degradation II	2	1	1
γ-linolenate biosynthesis II (animals)	2	1	1
pseudouridine degradation	2	1	1
pentose phosphate pathway (oxidative branch) I	3	3	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
L-alanine degradation II (to D-lactate)	3	2	1
3-methyl-branched fatty acid α-oxidation	6	3	2
mannitol biosynthesis	3	1	1
ethene biosynthesis IV (engineered)	3	1	1
oleate biosynthesis I (plants)	3	1	1
alkane biosynthesis II	3	1	1
GDP-mannose biosynthesis	4	4	1
phytol degradation	4	3	1
mannitol degradation II	4	2	1
long chain fatty acid ester synthesis (engineered)	4	1	1
phosphatidylcholine acyl editing	4	1	1
wax esters biosynthesis II	4	1	1
sporopollenin precursors biosynthesis	18	4	4
octane oxidation	5	4	1
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
1,5-anhydrofructose degradation	5	2	1
fatty acid salvage	6	6	1
stearate biosynthesis II (bacteria and plants)	6	5	1
stearate biosynthesis IV	6	4	1
6-gingerol analog biosynthesis (engineered)	6	3	1
stearate biosynthesis I (animals)	6	1	1
capsaicin biosynthesis	7	3	1
L-glutamate degradation XI (reductive Stickland reaction)	7	3	1
ceramide degradation by α-oxidation	7	2	1
4-aminobutanoate degradation V	7	2	1
β-(1,4)-mannan degradation	7	2	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
pentose phosphate pathway	8	8	1
2-deoxy-D-ribose degradation II	8	6	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
L-glutamate degradation V (via hydroxyglutarate)	10	6	1
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
colanic acid building blocks biosynthesis	11	9	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	1	1
superpathway of glucose and xylose degradation	17	15	1
methylaspartate cycle	19	10	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	2
palmitate biosynthesis III	29	28	1
oleate β-oxidation	35	30	1