Experiment set9IT026 for Bacteroides thetaiotaomicron VPI-5482

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BHIS with Cefoxitin sodium salt 0.025 mM

Group: stress
Media: BHIS + Cefoxitin sodium salt (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 4 genes in this experiment

For stress Cefoxitin sodium salt in Bacteroides thetaiotaomicron VPI-5482

For stress Cefoxitin sodium salt across organisms

SEED Subsystems

Subsystem #Specific
Glycolysis and Gluconeogenesis 2
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 1
nitrogen remobilization from senescing leaves 8 5 1
sucrose biosynthesis I (from photosynthesis) 9 6 1
glycolysis II (from fructose 6-phosphate) 11 10 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 5 1
gluconeogenesis III 12 10 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 10 1
glycolysis I (from glucose 6-phosphate) 13 11 1
gluconeogenesis I 13 11 1
Calvin-Benson-Bassham cycle 13 9 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 7 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
oxygenic photosynthesis 17 9 1
superpathway of hexitol degradation (bacteria) 18 12 1
superpathway of anaerobic sucrose degradation 19 14 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of N-acetylneuraminate degradation 22 16 1
ethene biosynthesis V (engineered) 25 15 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 18 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 12 1
1-butanol autotrophic biosynthesis (engineered) 27 12 1