Experiment set9IT022 for Synechococcus elongatus PCC 7942

Compare to:

Time 0 for outgrown library 2.0

Group: lib 6.2 test
Media: BG11, pH=7.5
Culturing: SynE_ML6.2, 100ml in 250 ml Flask, Aerobic, at 30 (C), shaken=0 rpm, (Solid)
By: Ben Rubin on 8/21/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 14 genes in this experiment

SEED Subsystems

Subsystem #Specific
Carotenoids 1
De Novo Purine Biosynthesis 1
Phycobilisome 1
Streptococcus pyogenes recombinatorial zone 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate 2 2 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
trans-lycopene biosynthesis II (oxygenic phototrophs and green sulfur bacteria) 8 6 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of carotenoid biosynthesis in plants 22 8 1