Experiment set9IT022 for Desulfovibrio vulgaris Miyazaki F

Compare to:

MoYLS4 media with N2

Group: nitrogen source
Media: MoLS4_no_ammonia + N2 (90 % atm), pH=7.2
Culturing: Miya_ML3_a, SerumBottle, Anaerobic, at 30 (C), shaken=0 rpm
By: Wetmore (Baker) on 22-Apr-19
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 1 genes in this experiment

For nitrogen source N2 in Desulfovibrio vulgaris Miyazaki F

For nitrogen source N2 across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.