Experiment set9IT019 for Synechococcus elongatus PCC 7942

No grazer plate rep 1

Group: grazers
Media: BG11, pH=7.5
Culturing: SynE_ML6, Petri dish, Aerobic, at 30 (C), shaken=0 rpm, (Solid)
By: Ben Rubin on 8/21/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 15 genes in this experiment

SEED Subsystems

Subsystem	#Specific
DNA Repair Base Excision	1
DNA repair, bacterial RecFOR pathway	1
High affinity phosphate transporter and control of PHO regulon	1
Phosphate metabolism	1
Purine conversions	1
Streptococcal Mga Regulon	1
Two-component regulatory systems in Campylobacter	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• Pyrimidine metabolism
• Glutamate metabolism
• Starch and sucrose metabolism
• Aminosugars metabolism
• Lipopolysaccharide biosynthesis
• Ether lipid metabolism
• Sphingolipid metabolism
• Thiamine metabolism
• Riboflavin metabolism
• Nicotinate and nicotinamide metabolism
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine nucleobases salvage I	1	1	1
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
pyrimidine nucleobases salvage II	2	2	1
ammonia assimilation cycle III	3	3	1
sucrose biosynthesis III	3	2	1
adenine and adenosine salvage V	3	1	1
purine deoxyribonucleosides degradation II	3	1	1
superpathway of pyrimidine nucleobases salvage	4	4	1
guanosine ribonucleotides de novo biosynthesis	4	4	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
purine deoxyribonucleosides degradation I	4	1	1
adenine and adenosine salvage III	4	1	1
adenosine nucleotides degradation II	5	2	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
purine ribonucleosides degradation	6	1	1
L-glutamate and L-glutamine biosynthesis	7	4	1
superpathway of purine deoxyribonucleosides degradation	7	2	1
superpathway of guanosine nucleotides de novo biosynthesis II	8	7	1
sucrose biosynthesis II	8	6	1
L-citrulline biosynthesis	8	5	1
sucrose biosynthesis I (from photosynthesis)	9	7	1
superpathway of pyrimidine ribonucleosides salvage	10	7	1
purine nucleotides degradation II (aerobic)	11	5	1
superpathway of L-citrulline metabolism	12	7	1
superpathway of purine nucleotide salvage	14	10	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of purine nucleotides de novo biosynthesis II	26	24	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	43	1