Experiment set9IT010 for Paraburkholderia graminis OAS925

Compare to:

Acetylated xylan 10 g/L carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Acetylated xylan (10 g/L)
Culturing: Burkholderia_OAS925_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Marta Torres on 4-Jul-21
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 29 genes in this experiment

For carbon source Acetylated xylan in Paraburkholderia graminis OAS925

For carbon source Acetylated xylan across organisms

SEED Subsystems

Subsystem #Specific
Alkanesulfonate assimilation 1
Arginine Biosynthesis extended 1
Glutathione: Non-redox reactions 1
Glycerol and Glycerol-3-phosphate Uptake and Utilization 1
Glycerol fermenation to 1,3-propanediol 1
Heat shock dnaK gene cluster extended 1
Methylcitrate cycle 1
Methylglyoxal Metabolism 1
Oxidative stress 1
Phosphate metabolism 1
Photorespiration (oxidative C2 cycle) 1
Propionate-CoA to Succinate Module 1
Protein degradation 1
Purine conversions 1
Ribosome biogenesis bacterial 1
Stringent Response, (p)ppGpp metabolism 1
Transport of Manganese 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosine nucleotides degradation III 1 1 1
superoxide radicals degradation 2 2 1
methanol oxidation to formaldehyde IV 2 1 1
pseudouridine degradation 2 1 1
ethanol degradation IV 3 3 1
methylglyoxal degradation VIII 3 3 1
ppGpp metabolism 6 5 2
methylglyoxal degradation I 3 2 1
reactive oxygen species degradation 4 4 1
2-methylcitrate cycle I 5 5 1
L-ornithine biosynthesis I 5 5 1
L-tryptophan biosynthesis 6 6 1
superpathway of methylglyoxal degradation 8 4 1
L-arginine biosynthesis I (via L-ornithine) 9 9 1
L-arginine biosynthesis III (via N-acetyl-L-citrulline) 9 8 1
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of arginine and polyamine biosynthesis 17 14 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of chorismate metabolism 59 42 1