Experiment set9IT006 for Pseudomonas fluorescens SBW25-INTG

Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs

Group: soil
Media: + Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
Culturing: PseudoSBW25_INTG_ML3, at 21 (C)
By: Joshua Elmore on 13-Feb-20

Specific Phenotypes

For 13 genes in this experiment

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs in Pseudomonas fluorescens SBW25-INTG

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs across organisms

SEED Subsystems

Subsystem	#Specific
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Homogentisate pathway of aromatic compound degradation	1
Isoleucine degradation	1
Leucine Degradation and HMG-CoA Metabolism	1
Methylcitrate cycle	1
Potassium homeostasis	1
Propionate-CoA to Succinate Module	1
Salicylate and gentisate catabolism	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutamate metabolism
• Valine, leucine and isoleucine degradation
• Arginine and proline metabolism
• Tyrosine metabolism
• Propanoate metabolism
• Styrene degradation
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
cis-cyclopropane fatty acid (CFA) biosynthesis	1	1	1
L-glutamate degradation I	1	1	1
sterculate biosynthesis	2	1	1
L-alanine degradation II (to D-lactate)	3	3	1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA	3	3	1
ethene biosynthesis IV (engineered)	3	1	1
queuosine biosynthesis I (de novo)	4	4	1
2-methylcitrate cycle I	5	5	1
L-tyrosine degradation I	5	5	1
2-methylcitrate cycle II	6	5	1
L-glutamate degradation XI (reductive Stickland reaction)	7	3	1
4-aminobutanoate degradation V	7	3	1
L-glutamate degradation V (via hydroxyglutarate)	10	6	1
methylaspartate cycle	19	9	1
mycolate biosynthesis	205	20	3
superpathway of mycolate biosynthesis	239	21	3