Experiment set9H20 for Pseudomonas stutzeri RCH2
Nitrite/ lactate media with Nitrite 20mM
Group: anaerobicMedia: RCH2_Lactate_noTEA_YE_C + Sodium nitrite (20 mM), pH=7
Culturing: psRCH2_ML7d, hungate, Anaerobic, at 30 (C), shaken=200 rpm
Growth: about 4.4 generations
By: Mike Adams on 12/17/2013
Specific Phenotypes
For 6 genes in this experiment
For anaerobic Sodium nitrite in Pseudomonas stutzeri RCH2
For anaerobic Sodium nitrite across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Denitrification | 5 |
| Dissimilatory nitrite reductase | 2 |
| Coenzyme B12 biosynthesis | 1 |
| Experimental tye | 1 |
| Heme and Siroheme Biosynthesis | 1 |
| Heme biosynthesis orphans | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| siroheme biosynthesis | 4 | 4 | 3 |
| factor 430 biosynthesis | 7 | 3 | 3 |
| nitrate reduction I (denitrification) | 5 | 4 | 2 |
| nitrate reduction VII (denitrification) | 5 | 3 | 2 |
| nitrifier denitrification | 6 | 3 | 2 |
| nitrite-dependent anaerobic methane oxidation | 3 | 1 | 1 |
| ammonia oxidation II (anaerobic) | 3 | 1 | 1 |
| cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) | 15 | 4 | 3 |
| cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) | 13 | 3 | 2 |
| adenosylcobalamin biosynthesis I (anaerobic) | 36 | 20 | 3 |
| adenosylcobalamin biosynthesis II (aerobic) | 33 | 21 | 2 |