Experiment set8S8 for Pantoea sp. MT58

Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50

Group: survival
Media: + Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Culturing: MT058_ML2, tube, Aerobic
By: Mike Thorgersen on 6/27/23

Specific Phenotypes

For 11 genes in this experiment

For survival Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 in Pantoea sp. MT58

For survival Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 across organisms

SEED Subsystems

Subsystem	#Specific
D-gluconate and ketogluconates metabolism	3
Glycine and Serine Utilization	2
Photorespiration (oxidative C2 cycle)	2
Allantoin Utilization	1
D-galactarate, D-glucarate and D-glycerate catabolism	1
Fermentations: Lactate	1
Fermentations: Mixed acid	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Glycine cleavage system	1
Mannitol Utilization	1
Nitrosative stress	1
Oxidative stress	1
Rrf2 family transcriptional regulators	1
Serine-glyoxylate cycle	1
Transport of Manganese	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Pentose phosphate pathway
• Glycerolipid metabolism
• Pyruvate metabolism
• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to (R)-lactate	1	1	1
2-O-α-mannosyl-D-glycerate degradation	2	1	1
L-alanine degradation II (to D-lactate)	3	3	1
glycine biosynthesis II	3	3	1
glycine degradation	3	3	1
glycine cleavage	3	3	1
vancomycin resistance I	3	2	1
D-galactarate degradation I	4	4	1
D-glucarate degradation I	4	4	1
glycolate and glyoxylate degradation I	4	3	1
photorespiration III	9	5	2
photorespiration I	9	5	2
superpathway of D-glucarate and D-galactarate degradation	5	5	1
glucose degradation (oxidative)	5	4	1
photorespiration II	10	6	2
L-alanine degradation VI (reductive Stickland reaction)	6	2	1
superpathway of glycol metabolism and degradation	7	6	1
L-ascorbate biosynthesis VIII (engineered pathway)	7	5	1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)	8	4	1
2-deoxy-D-ribose degradation II	8	3	1
folate transformations III (E. coli)	9	9	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	7	1
Entner-Doudoroff pathway II (non-phosphorylative)	9	6	1
reductive glycine pathway of autotrophic CO2 fixation	9	5	1
superpathway of L-alanine fermentation (Stickland reaction)	9	4	1
folate transformations II (plants)	11	10	1
folate transformations I	13	9	1
formaldehyde assimilation I (serine pathway)	13	8	1
mixed acid fermentation	16	14	1
superpathway of glucose and xylose degradation	17	16	1
heterolactic fermentation	18	16	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	19	1