Experiment set8S6 for Pantoea sp. MT58

Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50

Group: survival
Media: + Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Culturing: MT058_ML2, tube, Aerobic
By: Mike Thorgersen on 06/27/2023

Specific Phenotypes

For 13 genes in this experiment

For survival Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 in Pantoea sp. MT58

For survival Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 5 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 across organisms

SEED Subsystems

Subsystem	#Specific
Glycine and Serine Utilization	2
Glycine cleavage system	2
Photorespiration (oxidative C2 cycle)	2
Cobalt-zinc-cadmium resistance	1
Fermentations: Lactate	1
Fermentations: Mixed acid	1
Lysine Biosynthesis DAP Pathway	1
Mannitol Utilization	1
Methionine Biosynthesis	1
Nitrosative stress	1
Oxidative stress	1
Rrf2 family transcriptional regulators	1
Threonine and Homoserine Biosynthesis	1
Transport of Manganese	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• Biosynthesis of plant hormones
• Pyruvate metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to (R)-lactate	1	1	1
glycine biosynthesis II	3	3	2
glycine cleavage	3	3	2
L-homoserine biosynthesis	3	3	2
superpathway of L-threonine biosynthesis	6	6	2
L-alanine degradation II (to D-lactate)	3	3	1
vancomycin resistance I	3	2	1
superpathway of L-homoserine and L-methionine biosynthesis	8	8	2
dipicolinate biosynthesis	4	3	1
L-methionine biosynthesis IV	4	2	1
spermidine biosynthesis II	4	2	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	9	2
superpathway of L-methionine biosynthesis (transsulfuration)	9	9	2
ectoine biosynthesis	5	3	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	10	2
norspermidine biosynthesis	6	4	1
L-alanine degradation VI (reductive Stickland reaction)	6	2	1
superpathway of L-isoleucine biosynthesis I	13	13	2
L-lysine biosynthesis VI	7	6	1
L-lysine biosynthesis III	7	6	1
3-dehydroquinate biosynthesis II (archaea)	7	3	1
cremeomycin biosynthesis	7	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
superpathway of polyamine biosynthesis III	8	4	1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)	8	4	1
grixazone biosynthesis	8	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	2
L-lysine biosynthesis I	9	9	1
L-lysine biosynthesis II	9	7	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	7	1
superpathway of L-alanine fermentation (Stickland reaction)	9	4	1
chorismate biosynthesis II (archaea)	12	8	1
aspartate superpathway	25	24	2
mixed acid fermentation	16	14	1
superpathway of glucose and xylose degradation	17	16	1
heterolactic fermentation	18	16	1
platensimycin biosynthesis	26	6	1