Experiment set8S22 for Pantoea sp. MT58

Survival; Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50

Group: survival
Media: + Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50
Culturing: MT058_ML2, tube, Aerobic
By: Mike Thorgersen on 06/27/2023

Specific Phenotypes

For 20 genes in this experiment

For survival Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 in Pantoea sp. MT58

For survival Treatment=FW300 (pH7.4) with 1 mM Glucose and 0 mM NaH2PO4 and 2 mg/mL CCCP; TreatmentDuration=24hrs; Outgrowth=LBkan50 across organisms

SEED Subsystems

Subsystem	#Specific
Calvin-Benson cycle	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
LOS core oligosaccharide biosynthesis	1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
Pentose phosphate pathway	1
Sucrose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Carotenoid biosynthesis - General
• Anthocyanin biosynthesis
• Pentose phosphate pathway
• Fructose and mannose metabolism
• Fatty acid biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• High-mannose type N-glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Keratan sulfate biosynthesis
• Peptidoglycan biosynthesis
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• Ether lipid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosphingolipid biosynthesis - globo series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• Carbon fixation in photosynthetic organisms
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Zeatin biosynthesis
• Flavonoid biosynthesis
• Flavone and flavonol biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of ansamycins
• Biosynthesis of type II polyketide backbone
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pentose phosphate pathway (partial)	3	3	2
pentose phosphate pathway (non-oxidative branch) I	5	5	2
pentose phosphate pathway (non-oxidative branch) II	6	5	2
oleate biosynthesis III (cyanobacteria)	3	2	1
D-apiose degradation I	3	2	1
pentose phosphate pathway	8	8	2
CDP-diacylglycerol biosynthesis II	4	4	1
CDP-diacylglycerol biosynthesis I	4	4	1
formaldehyde assimilation II (assimilatory RuMP Cycle)	9	8	2
Rubisco shunt	10	9	2
phosphatidate biosynthesis (yeast)	5	3	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	2
phosphatidylglycerol biosynthesis I	6	6	1
phosphatidylglycerol biosynthesis II	6	6	1
formaldehyde assimilation III (dihydroxyacetone cycle)	12	11	2
superpathway of stearidonate biosynthesis (cyanobacteria)	6	2	1
palmitoyl ethanolamide biosynthesis	6	2	1
Calvin-Benson-Bassham cycle	13	10	2
diacylglycerol and triacylglycerol biosynthesis	7	3	1
stigma estolide biosynthesis	7	2	1
anandamide biosynthesis II	8	2	1
superpathway of glucose and xylose degradation	17	16	2
oxygenic photosynthesis	17	11	2
lipid A-core biosynthesis (E. coli K-12)	10	4	1
lipid A-core biosynthesis (Salmonella)	10	3	1
anandamide biosynthesis I	12	4	1
ethene biosynthesis V (engineered)	25	18	2
superpathway of cardiolipin biosynthesis (bacteria)	13	11	1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	19	2
1-butanol autotrophic biosynthesis (engineered)	27	19	2
superpathway of phospholipid biosynthesis II (plants)	28	10	2
Bifidobacterium shunt	15	14	1
superpathway of Kdo2-lipid A biosynthesis	25	19	1