Experiment set8S1 for Pantoea sp. MT58

Survival; Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50

Group: survival
Media: + Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50
Culturing: MT058_ML2, tube, Aerobic
By: Mike Thorgersen on 6/27/23

Specific Phenotypes

For 8 genes in this experiment

For survival Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 in Pantoea sp. MT58

For survival Treatment=UGA synthetic gw pH7.4 with 1 mM Glucose and 0 mM NaH2PO4; TreatmentDuration=24hrs; Outgrowth=LBkan50 across organisms

SEED Subsystems

Subsystem	#Specific
Glycine and Serine Utilization	2
Glycine cleavage system	2
Photorespiration (oxidative C2 cycle)	2
Beta-Glucoside Metabolism	1
Cobalt-zinc-cadmium resistance	1
Nitrosative stress	1
Oxidative stress	1
Rrf2 family transcriptional regulators	1
Transport of Manganese	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Cyanoamino acid metabolism
• Starch and sucrose metabolism
• Phenylpropanoid biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine cleavage	3	3	2
glycine biosynthesis II	3	3	2
neolinustatin bioactivation	3	2	2
linustatin bioactivation	4	2	2
lotaustralin degradation	2	1	1
linamarin degradation	2	1	1
cellulose degradation II (fungi)	3	2	1
coumarin biosynthesis (via 2-coumarate)	5	2	1
α-tomatine degradation	6	1	1
firefly bioluminescence	14	2	1