Experiment set8IT095 for Phaeobacter inhibens DSM 17395

Compare to:

marine broth with Choline Lysine 10 mM

Group: stress
Media: marine_broth_2216 + Choline Lysine (10 mM)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Mark on 1/20/2015
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 1094 F1,F2

Specific Phenotypes

For 6 genes in this experiment

For stress Choline Lysine in Phaeobacter inhibens DSM 17395

For stress Choline Lysine across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 3
Choline Transport 1
Terminal cytochrome C oxidases 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
choline-O-sulfate degradation 3 3 3
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 2
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 2
choline degradation I 2 2 2
arsenite to oxygen electron transfer 2 1 1
β-alanine biosynthesis IV 2 1 1
β-alanine biosynthesis I 2 1 1
glycine betaine biosynthesis III (plants) 3 2 1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia) 3 1 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
aerobic respiration I (cytochrome c) 4 3 1
choline degradation IV 4 2 1
aerobic respiration II (cytochrome c) (yeast) 4 2 1
dimethylsulfoniopropanoate biosynthesis II (Spartina) 4 1 1
Fe(II) oxidation 6 2 1