Experiment set8IT095 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with 8-hydroxy-nitroquinoline 0.05 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + 8-hydroxy-nitroquinoline (0.05 mM) + Dimethyl Sulfoxide (0.5 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 5 genes in this experiment

For stress 8-hydroxy-nitroquinoline in Bacteroides thetaiotaomicron VPI-5482

For stress 8-hydroxy-nitroquinoline across organisms

SEED Subsystems

Subsystem	#Specific
Transport of Zinc	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.