Experiment set8IT084 for Pseudomonas fluorescens FW300-N2C3

50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride

Group: resistance
Media: RCH2_defined_putrescine_20mM + P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride (50 percent), pH=7
Culturing: pseudo5_N2-C3_1_ML2B, 15 mL tube, aerobic, at 30 (C), shaken=225 rpm
By: Lauren_Andrew on 12/2/2016

Specific Phenotypes

For 23 genes in this experiment

For resistance P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride in Pseudomonas fluorescens FW300-N2C3

For resistance P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine dihydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter dipeptide (TC 3.A.1.5.2)	6
Bacterial Chemotaxis	2
Alanine biosynthesis	1
Lysine degradation	1
Polyamine Metabolism	1
Protein degradation	1
Pyruvate Alanine Serine Interconversions	1
SigmaB stress responce regulation	1
Universal stress protein family	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Alanine and aspartate metabolism
• Lysine degradation
• beta-Alanine metabolism
• Propanoate metabolism
• Butanoate metabolism
• Fatty acid biosynthesis
• Methionine metabolism
• Valine, leucine and isoleucine degradation
• Lysine biosynthesis
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Aminophosphonate metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Thiamine metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Caprolactam degradation
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of terpenoids and steroids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine biosynthesis III	1	1	1
L-glutamine biosynthesis I	1	1	1
L-cysteine degradation IV	1	1	1
putrescine degradation II	4	4	2
β-alanine degradation II	2	2	1
putrescine degradation V	2	2	1
4-aminobutanoate degradation III	2	2	1
ammonia assimilation cycle I	2	2	1
4-aminobutanoate degradation II	2	2	1
ammonia assimilation cycle II	2	2	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
4-aminobutanoate degradation I	2	1	1
ammonia assimilation cycle III	3	3	1
superpathway of ammonia assimilation (plants)	3	3	1
L-aspartate degradation II (aerobic)	3	2	1
superpathway of 4-aminobutanoate degradation	3	2	1
L-aspartate degradation III (anaerobic)	3	2	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
Arg/N-end rule pathway (eukaryotic)	14	8	4
thiazole component of thiamine diphosphate biosynthesis II	7	4	2
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	10	3
superpathway of ornithine degradation	8	8	2
superpathway of L-alanine biosynthesis	4	4	1
GABA shunt II	4	2	1
GABA shunt I	4	1	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
superpathway of L-arginine and L-ornithine degradation	13	12	3
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
superpathway of thiamine diphosphate biosynthesis II	11	8	2
β-alanine biosynthesis II	6	5	1
molybdopterin biosynthesis	6	4	1
thiazole component of thiamine diphosphate biosynthesis I	6	3	1
L-glutamate and L-glutamine biosynthesis	7	6	1
4-aminobutanoate degradation V	7	2	1
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
L-arginine biosynthesis II (acetyl cycle)	10	10	1
superpathway of coenzyme A biosynthesis II (plants)	10	9	1
superpathway of thiamine diphosphate biosynthesis I	10	7	1
tRNA-uridine 2-thiolation and selenation (bacteria)	11	4	1
nicotine degradation I (pyridine pathway)	17	7	1