Experiment set8IT080 for Klebsiella michiganensis M5al

Compare to:

RCH2_defined_glucose with MS_media 0.5x

Group: carbon source
Media: RCH2_defined_glucose + 0.5x MS_media, pH=7
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

MS_media 0.5x includes: 825 mg/L Ammonium Nitrate, 3.1 mg/L Boric Acid, 166.1 mg/L Calcium chloride, 0.0125 mg/L Cobalt chloride hexahydrate, 0.0125 mg/L Copper (II) sulfate pentahydrate, 18.63 mg/L EDTA (disodium salt), 13.9 mg/L Iron (II) sulfate heptahydrate, 1 mg/L Glycine, 90.35 mg/L Magnesium sulfate, 8.45 mg/L Manganese sulfate, 50 mg/L m-Inositol, 0.25 mg/L Nicotinic Acid, 0.415 mg/L Potassium iodide, 950 mg/L Potassium nitrate, 85 mg/L Potassium phosphate monobasic, 0.25 mg/L Pyridoxine HCl, 0.125 mg/L Sodium Molybdate Dihydrate, 0.05 mg/L Thiamine HCl, 4.3 mg/L Zinc sulfate heptahydrate, 10 g/L Sucrose (final concentrations)

Specific Phenotypes

For 2 genes in this experiment

For carbon source MS_media in Klebsiella michiganensis M5al

For carbon source MS_media across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.