Experiment set8IT076 for Klebsiella michiganensis M5al

Compare to:

Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.33 x) + D-Glucose (74 mM)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate

Specific Phenotypes

For 5 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem #Specific
Biotin biosynthesis 1
Methionine Biosynthesis 1
Multidrug Resistance Efflux Pumps 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 2
biotin biosynthesis from 8-amino-7-oxononanoate II 4 3 2
biotin biosynthesis from 8-amino-7-oxononanoate III 5 3 2
S-adenosyl-L-methionine salvage II 3 3 1
biotin biosynthesis II 6 4 2
S-adenosyl-L-methionine salvage I 4 4 1
L-methionine biosynthesis III 4 2 1
L-methionine biosynthesis I 5 5 1
seleno-amino acid biosynthesis (plants) 5 3 1
thiazole component of thiamine diphosphate biosynthesis I 6 6 1
L-methionine biosynthesis II 6 5 1
biotin biosynthesis I 15 15 2
superpathway of L-homoserine and L-methionine biosynthesis 8 8 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 9 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 9 1
superpathway of thiamine diphosphate biosynthesis I 10 10 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 10 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 18 1
superpathway of seleno-compound metabolism 19 6 1
aspartate superpathway 25 24 1