Experiment set8IT074 for Klebsiella michiganensis M5al

Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1_noCarbon + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.67 x) + D-Glucose (37 mM)
Culturing: Koxy_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 28-Feb-18
Media components: 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate

Specific Phenotypes

For 7 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem	#Specific
Lipid A-Ara4N pathway ( Polymyxin resistance )	2
Multidrug Resistance Efflux Pumps	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Lipopolysaccharide biosynthesis
• Puromycin biosynthesis
• Methionine metabolism
• Lysine biosynthesis
• Lysine degradation
• Arginine and proline metabolism
• Aminophosphonate metabolism
• Nucleotide sugars metabolism
• Peptidoglycan biosynthesis
• Caprolactam degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
polymyxin resistance	6	5	5
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
juvenile hormone III biosynthesis I	6	1	1