Experiment set8IT070 for Synechococcus elongatus PCC 7942

BG11 with Sodium Chloride 250 mM

Group: stress
Media: BG11 + Sodium Chloride (250 mM)
Culturing: SynE_ML6, 12 well microplate, Aerobic, at 30 (C), shaken=0 rpm
By: Mark on 10/2/2015
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)

Specific Phenotypes

For 22 genes in this experiment

For stress Sodium Chloride in Synechococcus elongatus PCC 7942

For stress Sodium Chloride across organisms

SEED Subsystems

Subsystem	#Specific
Synechocystis experimental	2
Campylobacter Iron Metabolism	1
Iron acquisition in Vibrio	1
Pyruvate Alanine Serine Interconversions	1
RNA processing and degradation, bacterial	1
Soluble cytochromes and functionally related electron carriers	1
Transport of Iron	1
Two cell division clusters relating to chromosome partitioning	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Glutamate metabolism
• Taurine and hypotaurine metabolism
• Starch and sucrose metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine degradation IV	1	1	1
L-glutamine degradation I	1	1	1
L-glutamate biosynthesis I	2	2	1
ammonia assimilation cycle III	3	3	1
sucrose biosynthesis III	3	2	1
L-asparagine biosynthesis III (tRNA-dependent)	4	4	1
glutaminyl-tRNAgln biosynthesis via transamidation	4	4	1
guanosine ribonucleotides de novo biosynthesis	4	4	1
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
L-glutamate and L-glutamine biosynthesis	7	4	1
superpathway of guanosine nucleotides de novo biosynthesis II	8	7	1
sucrose biosynthesis II	8	6	1
L-citrulline biosynthesis	8	5	1
sucrose biosynthesis I (from photosynthesis)	9	7	1
superpathway of L-citrulline metabolism	12	7	1
superpathway of purine nucleotide salvage	14	10	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	1
superpathway of purine nucleotides de novo biosynthesis II	26	24	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	43	1