Experiment set8IT067 for Bacteroides thetaiotaomicron VPI-5482

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BHIS with Vancomycin Hydrochloride from Streptomyces orientalis 0.0125 mM

Group: stress
Media: BHIS + Vancomycin Hydrochloride from Streptomyces orientalis (0.0125 mM) + Dimethyl Sulfoxide (0.125 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 9 genes in this experiment

For stress Vancomycin Hydrochloride from Streptomyces orientalis in Bacteroides thetaiotaomicron VPI-5482

For stress Vancomycin Hydrochloride from Streptomyces orientalis across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 1
Peptidoglycan Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
sulfate activation for sulfonation 2 2 1
assimilatory sulfate reduction III 3 1 1
assimilatory sulfate reduction II 3 1 1
sulfite oxidation III 3 1 1
assimilatory sulfate reduction I 4 2 1
assimilatory sulfate reduction IV 4 2 1
selenate reduction 5 2 1
dissimilatory sulfate reduction I (to hydrogen sufide)) 5 1 1
peptidoglycan maturation (meso-diaminopimelate containing) 12 4 2
superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) 6 1 1
superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) 6 1 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 7 1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 4 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 8 1
superpathway of sulfide oxidation (phototrophic sulfur bacteria) 12 2 1
peptidoglycan biosynthesis II (staphylococci) 17 12 1
peptidoglycan biosynthesis IV (Enterococcus faecium) 17 12 1
superpathway of seleno-compound metabolism 19 5 1